Newly identified DNA methyltransferases of Ixodes ricinus ticks
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31812591
DOI
10.1016/j.ttbdis.2019.101348
PII: S1877-959X(19)30199-2
Knihovny.cz E-zdroje
- Klíčová slova
- 5-Methylcytosine, DNA methylation, Epigenetics, Ixodes tick, Methyltransferase, N6-Methyladenine,
- MeSH
- epigeneze genetická * MeSH
- genetická transkripce MeSH
- klíště enzymologie genetika růst a vývoj MeSH
- larva genetika růst a vývoj MeSH
- methyltransferasy chemie genetika MeSH
- molekulární konformace MeSH
- nymfa genetika růst a vývoj MeSH
- ovum růst a vývoj MeSH
- proteiny členovců chemie genetika MeSH
- regulace genové exprese * MeSH
- transkriptom * MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- methyltransferasy MeSH
- proteiny členovců MeSH
DNA methylation at the fifth position of cytosine (5mC) and at the sixth position of adenine (6 mA) plays an important role in the regulation of the gene expression and, in eukaryotes, is essential for normal development. For Ixodes ricinus, the most common European arthropod vector of human and animal pathogens, the DNA methylation profile and the role of DNA methylation in tick development are still under discussion. Our goal was to analyze the status of I. ricinus DNA methylation at different life stages and identify enzymes that produce this type of DNA modification. We found that 5mC and 6mA are present in I. ricinus genomic DNA at all life stages. In the transcriptome of I. ricinus, we identified the sequences of the putative IrDNMT1, IrDNMT3, and IrDAMT enzymes, and bioinformatic analysis and three-dimensional modeling predicted their DNA methylation activity. This confirms that I. ricinus possesses a complete DNA methylation toolkit. Our results suggest that DNA methylation is important for the physiology and transstadial development of ticks.
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