Heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin (2) and cholesterol form a water-soluble complex 3. We performed several NMR studies, particularly 1H, 13C, 2D NOESY and DOSY, at various temperatures on 500 and 950 MHz instruments. We discovered that the complex 3 is unstable above 57 °C in heavy water, while it is kinetically stable enough to be studied by NMR in detail at 1 °C. We demonstrated the formation of a face-to-face 2:1 complex with a binding constant of approximately 2.2 × 106 M-2.

University of Chemistry and Technology Prague Department of Analytical Chemistry Technická 5 166 28 Prague 6 Czech Republic

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