Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31874193
DOI
10.1016/j.molbiopara.2019.111248
PII: S0166-6851(19)30163-X
Knihovny.cz E-resources
- Keywords
- Cystatin, Cytokines, IL-10, Immunomodulation, Monogenea, TNF-α,
- MeSH
- Cytokines * drug effects metabolism MeSH
- Immunomodulation MeSH
- Cysteine Proteinase Inhibitors pharmacology MeSH
- Interleukin-10 metabolism MeSH
- Carps parasitology MeSH
- Macrophages * drug effects metabolism MeSH
- Swine MeSH
- Recombinant Proteins pharmacology MeSH
- Tumor Necrosis Factor-alpha drug effects metabolism MeSH
- Trematoda immunology metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines * MeSH
- Cysteine Proteinase Inhibitors MeSH
- Interleukin-10 MeSH
- Recombinant Proteins MeSH
- Tumor Necrosis Factor-alpha MeSH
The gills of the common carp, whose mucosal surface belongs to the key defence mechanisms of piscine immunity, can be infested with both the larval and adult stage of Eudiplozoon nipponicum (Monogenea). Although on their own, monogeneans do not considerably compromise their hosts' health status, fish with epithelial barriers damaged in parasite feeding and attachment sites are at an increased risk of bacterial challenge with possible harmful consequences. Several studies suggest that helminth parasites of teleost fish evade and manipulate host immune system via their excretory-secretory products, but our knowledge of these processes in the monogeneans is limited. Cysteine peptidase inhibitors (CPI), which are found in the secretions of numerous parasites, often induce immunosuppression by subverting Th1 mechanisms and drawing the immune system towards a Th2/Treg response. We employed the qPCR to test the effect of recently characterised CPI of E. nipponicum (rEnStef) on the mRNA expression of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 produced by porcine macrophages in vitro. After an initial preincubation with rEnStef, we stimulated the macrophages using LPS. By inducing a Th1 pro-inflammatory response, we imitated the immune reaction during a bacterial challenge in tissue damaged by the feeding and attachment of E. nipponicum. We observed a significant dose-dependent downregulation of the expression of TNF-α and IL-10 cytokines. The observed suppression of TNF-alpha expression by rEnStef could result in decreased pathogen control, which might in turn lead to increased rates of secondary bacterial infections in fish infected by E. nipponicum.
Department of Botany and Zoology Faculty of Science Masaryk University Brno 611 37 Czech Republic
Department of Immunology Veterinary Research Institute Brno 621 00 Czech Republic
Department of Virology Veterinary Research Institute Brno 621 00 Czech Republic
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