Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
32000894
DOI
10.1016/bs.mie.2019.05.025
PII: S0076-6879(19)30184-3
Knihovny.cz E-zdroje
- Klíčová slova
- Calreticulin, Cancer prognosis, Flow cytometry, Immunogenic cell death, Immunohistochemistry,
- MeSH
- imunogenní buněčná smrt * MeSH
- imunohistochemie metody MeSH
- kalretikulin analýza imunologie MeSH
- lidé MeSH
- nádorové biomarkery analýza imunologie MeSH
- nádorové mikroprostředí MeSH
- nádory imunologie patologie MeSH
- průtoková cytometrie metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- kalretikulin MeSH
- nádorové biomarkery MeSH
Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.
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