Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
32000894
DOI
10.1016/bs.mie.2019.05.025
PII: S0076-6879(19)30184-3
Knihovny.cz E-resources
- Keywords
- Calreticulin, Cancer prognosis, Flow cytometry, Immunogenic cell death, Immunohistochemistry,
- MeSH
- Immunogenic Cell Death * MeSH
- Immunohistochemistry methods MeSH
- Calreticulin analysis immunology MeSH
- Humans MeSH
- Biomarkers, Tumor analysis immunology MeSH
- Tumor Microenvironment MeSH
- Neoplasms immunology pathology MeSH
- Flow Cytometry methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Calreticulin MeSH
- Biomarkers, Tumor MeSH
Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.
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