Multimodal and multiscale optical imaging of nanomedicine delivery across the blood-brain barrier upon sonopermeation
Jazyk angličtina Země Austrálie Médium electronic-ecollection
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32042346
PubMed Central
PMC6993230
DOI
10.7150/thno.41161
PII: thnov10p1948
Knihovny.cz E-zdroje
- Klíčová slova
- Blood-brain barrier, Drug delivery, Microbubbles, Nanomedicine, Ultrasound,
- MeSH
- fluorescenční barviva aplikace a dávkování MeSH
- hematoencefalická bariéra diagnostické zobrazování metabolismus MeSH
- liposomy aplikace a dávkování MeSH
- mikrobubliny MeSH
- mozek diagnostické zobrazování MeSH
- myši nahé MeSH
- myši MeSH
- nanomedicína metody MeSH
- nemoci mozku farmakoterapie MeSH
- optické zobrazování metody MeSH
- systémy cílené aplikace léků metody MeSH
- ultrasonografie metody MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fluorescenční barviva MeSH
- liposomy MeSH
Rationale: The blood-brain barrier (BBB) is a major obstacle for drug delivery to the brain. Sonopermeation, which relies on the combination of ultrasound and microbubbles, has emerged as a powerful tool to permeate the BBB, enabling the extravasation of drugs and drug delivery systems (DDS) to and into the central nervous system (CNS). When aiming to improve the treatment of high medical need brain disorders, it is important to systematically study nanomedicine translocation across the sonopermeated BBB. To this end, we here employed multimodal and multiscale optical imaging to investigate the impact of DDS size on brain accumulation, extravasation and penetration upon sonopermeation. Methods: Two prototypic DDS, i.e. 10 nm-sized pHPMA polymers and 100 nm-sized PEGylated liposomes, were labeled with fluorophores and intravenously injected in healthy CD-1 nude mice. Upon sonopermeation, computed tomography-fluorescence molecular tomography, fluorescence reflectance imaging, fluorescence microscopy, confocal microscopy and stimulated emission depletion nanoscopy were used to study the effect of DDS size on their translocation across the BBB. Results: Sonopermeation treatment enabled safe and efficient opening of the BBB, which was confirmed by staining extravasated endogenous IgG. No micro-hemorrhages, edema and necrosis were detected in H&E stainings. Multimodal and multiscale optical imaging showed that sonopermeation promoted the accumulation of nanocarriers in mouse brains, and that 10 nm-sized polymeric DDS accumulated more strongly and penetrated deeper into the brain than 100 nm-sized liposomes. Conclusions: BBB opening via sonopermeation enables safe and efficient delivery of nanomedicine formulations to and into the brain. When looking at accumulation and penetration (and when neglecting issues such as drug loading capacity and therapeutic efficacy) smaller-sized DDS are found to be more suitable for drug delivery across the BBB than larger-sized DDS. These findings are valuable for better understanding and further developing nanomedicine-based strategies for the treatment of CNS disorders.
Czech Academy of Sciences Institute of Macromolecular Chemistry Prague Czech Republic
Department of Targeted Therapeutics University of Twente Enschede The Netherlands
DWI Leibniz Institute for Interactive Materials RWTH Aachen University Aachen Germany
Fraunhofer MEVIS Institute for Medical Image Computing Aachen Germany
Institute of Organic and Macromolecular Chemistry Ulm University Ulm Germany
Institute of Pathology University Clinic RWTH Aachen Aachen Germany
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