The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
32113006
DOI
10.1016/j.jneuroim.2020.577191
PII: S0165-5728(19)30596-X
Knihovny.cz E-resources
- Keywords
- Concanavalin A, Morphine, Proteomic analysis, Rat spleen lymphocytes, Up-regulation of brain proteins,
- MeSH
- Lymphocyte Activation drug effects genetics MeSH
- Concanavalin A pharmacology MeSH
- Rats MeSH
- Lymphocytes drug effects metabolism MeSH
- Morphine pharmacology MeSH
- Rats, Wistar MeSH
- Proteome drug effects MeSH
- Proteomics methods MeSH
- Gene Expression Regulation drug effects MeSH
- Spleen cytology MeSH
- Gene Expression Profiling MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Concanavalin A MeSH
- Morphine MeSH
- Proteome MeSH
Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins; 101 proteins were down-regulated (>2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (>2-fold).
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