We performed micro-CT imaging of the vascular blood supply in the interscapular area of the brown adipose tissue in three mice with the use of intravascular contrast agent Aurovist™. Resulting 3D data rendering was then adapted into 2D resolution with visualization using false color system and grayscale images. These were then studied for the automatic quantification of the blood vessel density within this area. We found the highest most occurring density within arterioles or venules representing smaller blood vessels whereas with the increase of the vessel diameters a lower percentage rate of their presence was observed in the sample. Our study shows that micro-CT scanning in combination with Aurovist™ contrast is suitable for anatomical studies of interscapular area of brown adipose tissue blood vessel supply.

Centre of Scientific Information 3rd Faculty of Medicine Charles University Prague Czech Republic

Department of Anatomy 3rd Faculty of Medicine Charles University Prague Czech Republic

Department of Biosciences Vascular and Exercise Biology Unit University of Salzburg Austria

Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic

Institute of Experimental and Applied Physics Czech Technical University Prague Prague Czech Republic

Specialized Laboratory of Experimental Imaging of the 3rd Faculty of Medicine Charles University and the Institute of Experimental and Applied Physics and Faculty of Biomedical Engineering Czech Technical University Prague Prague Czech Republic

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