Ethanol fixation method for heart and lung imaging in micro-CT
Jazyk angličtina Země Japonsko Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
GAUK 130717
Grantová Agentura, Univerzita Karlova
No. CZ.02.1.01/0.0/0.0/16_019/0000766
European Regional Development Fund-Project: Engineering applications of microworld physics
PubMed
30875011
DOI
10.1007/s11604-019-00830-6
PII: 10.1007/s11604-019-00830-6
Knihovny.cz E-zdroje
- Klíčová slova
- Ethanol, Heart, Lungs, Mice, Micro-CT,
- MeSH
- ethanol * MeSH
- kontrastní látky * MeSH
- modely u zvířat MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- plíce anatomie a histologie diagnostické zobrazování MeSH
- rentgenová mikrotomografie metody MeSH
- srdce anatomie a histologie diagnostické zobrazování MeSH
- vylepšení obrazu metody MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ethanol * MeSH
- kontrastní látky * MeSH
PURPOSE: The soft tissue imaging in micro-CT remains challenging due to its low intrinsic contrast. The aim of this study was to create a simple staining method omitting the usage of contrast agents for ex vivo soft tissue imaging in micro-CT. MATERIALS AND METHODS: Hearts and lungs from 30 mice were used. Twenty-seven organs were either fixed in 97% or 50% ethanol solution or in a series of ascending ethanol concentrations. Images were acquired after 72, 168 and 336 h on a custom-built micro-CT machine and compared to scans of three native samples. RESULTS: Ethanol provided contrast enhancement in all evaluated fixations. Fixation in 97% ethanol resulted in contrast enhancement after 72 h; however, it caused hardening of the samples. Fixation in 50% ethanol provided contrast enhancement after 336 h, with milder hardening, compared to the 97% ethanol fixation, but the visualization of details was worse. The fixation in a series of ascending ethanol concentrations provided the most satisfactory results; all organs were visualized in great detail without tissue damage. CONCLUSIONS: Simple ethanol fixation improves the tissue contrast enhancement in micro-CT. The best results can be obtained with fixation of the soft tissue samples in a series of ascending ethanol concentrations.
Center for Experimental Biomodels 1st Faculty of Medicine Charles University Prague Czech Republic
Centre of Scientific Information 3rd Faculty of Medicine Charles University Prague Czech Republic
Faculty of Biomedical Engineering Czech Technical University Prague Kladno Czech Republic
Zobrazit více v PubMed
Circulation. 2002 Jan 15;105(2):E3-4 PubMed
Dev Dyn. 2006 Oct;235(10):2786-94 PubMed
BMC Physiol. 2009 Jun 22;9:11 PubMed
Circ Cardiovasc Imaging. 2010 May;3(3):314-22 PubMed
Invest Radiol. 2010 Jul;45(7):445-52 PubMed
J Appl Physiol (1985). 2010 Dec;109(6):1960-8 PubMed
Respir Res. 2010 Dec 22;11:181 PubMed
Micron. 2012 Feb;43(2-3):104-15 PubMed
J Anat. 2012 May;220(5):514-24 PubMed
J Microsc. 2013 Apr;250(1):21-31 PubMed
J Anat. 2013 Aug;223(2):185-93 PubMed
PLoS One. 2013 Dec 30;8(12):e84321 PubMed
PLoS One. 2014 Feb 10;9(2):e88129 PubMed
Methods. 2014 Nov;70(1):12-9 PubMed
Am J Physiol Lung Cell Mol Physiol. 2014 May 15;306(10):L897-914 PubMed
J Synchrotron Radiat. 2014 Jul;21(Pt 4):795-800 PubMed
Sci Rep. 2015 Sep 25;5:14088 PubMed
Dis Model Mech. 2016 Jan;9(1):91-8 PubMed
Sci Rep. 2016 Jul 27;6:30385 PubMed
