Skin wipe test: A simple, inexpensive, and fast approach in the diagnosis of cystic fibrosis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
32250037
DOI
10.1002/ppul.24758
Knihovny.cz E-zdroje
- Klíčová slova
- capillary electrophoresis, cystic fibrosis, diagnosis, ion ratio, screening, skin wipe test, sweat test,
- MeSH
- chloridy analýza MeSH
- cystická fibróza diagnóza MeSH
- diagnostické testy rutinní * MeSH
- dítě MeSH
- dospělí MeSH
- draslík analýza MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- pot chemie MeSH
- předškolní dítě MeSH
- senzitivita a specificita MeSH
- sodík analýza MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chloridy MeSH
- draslík MeSH
- sodík MeSH
OBJECTIVE: To assess the performance of a newly developed skin wipe test (SWT) for the diagnosis of cystic fibrosis (CF). STUDY DESIGN: Spontaneously formed sweat from the forearm was wiped by a cotton swab moistened with 100 µL of deionized (DI) water and extracted into 400 µL of DI water (SWT). The conventional Macroduct sweat test (ST) was performed simultaneously. SWT samples of 114 CF patients, 76 healthy carriers, and 58 controls were analyzed by capillary electrophoresis with contactless conductivity detection and Cl- /K+ and (Cl- + Na+ )/K+ ion ratios were evaluated. Chloride concentrations from Macroduct ST were analyzed coulometrically. RESULTS: Analysis of 248 SWT samples and simultaneous Macroduct ST samples showed comparable method performance. Two ion ratios, Cl- /K+ and (Cl- + Na+ )/K+ , from the SWT samples and Cl- values from the ST samples were evaluated to diagnose CF. Sensitivity of the SWT method using the Cl- /K+ ratio (cutoff value 3.9) was 93.9%, compared to 99.1% when using the (Cl- + Na+ )/K+ ratio (cutoff value 5.0) and 98.3% in using Macroduct Cl- (cutoff value higher or equal to 60 mmol/L). The methods' specificities were 97.8%, 94.0%, and 100.0%, respectively. CONCLUSIONS: The developed SWT method with capillary electrophoretic analysis for CF diagnosis performs comparably to the conventional Macroduct ST. The SWT method is simple, fast, inexpensive, and completely noninvasive. Use of an ion ratio in obtained SWT samples is proposed as a new diagnostic parameter that shows significant promise in CF diagnostics.
Bács Kiskun County Hospital and Teaching Hospital University of Szeged Kecskemét Hungary
Department of Bioanalytical Instrumentation CEITEC Masaryk University Brno Czech Republic
Department of Chemistry Masaryk University Brno Czech Republic
Department of Clinical Biochemistry University Hospital Brno Brno Czech Republic
Department of Medical Genetics University of Szeged Szeged Hungary
Department of Pediatrics Charles University and Motol University Hospital Prague Czech Republic
Department of Respiratory Diseases and TB University Hospital Brno Brno Czech Republic
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