BACKGROUND: Leishmaniasis is a human and animal disease caused by parasites of the genus Leishmania, which is now divided into four subgenera, Leishmania, Viannia, Sauroleishmania and Mundinia. Subgenus Mundinia, established in 2016, is geographically widely dispersed, its distribution covers all continents, except Antarctica. It consists of 5 species; L. enriettii and L. macropodum are parasites of wild mammals while L. martiniquensis, L. orientalis and an unnamed Leishmania sp. from Ghana are infectious to humans. There is very little information on natural reservoir hosts and vectors for any Mundinia species. METHODS: Experimental infections of guinea pigs with all five Mundinia species were performed. Animals were injected intradermally with 107 culture-derived promastigotes into both ear pinnae. The courses of infections were monitored weekly; xenodiagnoses were performed at weeks 4 and 8 post-infection using Lutzomyia migonei. The distribution of parasites in different tissues was determined post-mortem by conventional PCR. RESULTS: No significant differences in weight were observed between infected animals and the control group. Animals infected with L. enriettii developed temporary lesions at the site of inoculation and were infectious to Lu. migonei in xenodiagnoses. Animals infected with L. martiniquensis and L. orientalis developed temporary erythema and dry lesions at the site of inoculation, respectively, but were not infectious to sand flies. Guinea pigs infected by L. macropodum and Leishmania sp. from Ghana showed no signs of infection during experiments, were not infectious to sand flies and leishmanial DNA was not detected in their tissue samples at the end of experiments at week 12 post-inoculation. CONCLUSIONS: According to our results, guinea pigs are not an appropriate model organism for studying Mundinia species other than L. enriettii. We suggest that for better understanding of L. (Mundinia) biology it is necessary to focus on other model organisms.

Department of Parasitology Faculty of Science Charles University Prague Czech Republic

Division of Biomedical and Life Sciences Faculty of Health and Medicine Lancaster University Lancaster UK

Vector Biology and Vector Borne Disease Research Unit Department of Parasitology Faculty of Medicine Chulalongkorn University Bangkok Thailand

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Espinosa OA, Serrano MG, Camargo EP, Teixeira MMG, Shaw JJ. An appraisal of the taxonomy and nomenclature of trypanosomatids presently classified as Leishmania and Endotrypanum. Parasitology. 2016;145:430–442. doi: 10.1017/S0031182016002092. PubMed DOI

Barratt J, Kaufer A, Peters B, Craig D, Lawrence A, Roberts T, et al. Isolation of novel trypanosomatid, Zelonia australiensis sp. nov. (Kinetoplastida: Trypanosomatidae) provides support for a Gondwanan origin of dixenous parasitism in the Leishmaniinae. PLoS Negl Trop Dis. 2017;11:1–26. doi: 10.1371/journal.pntd.0005215. PubMed DOI PMC

Jariyapan N, Daroontum T, Jaiwong K, Chanmol W, Intakhan N, Sor-Suwan S, et al. Leishmania (Mundinia) orientalis n. sp. (Trypanosomatidae), a parasite from Thailand responsible for localised cutaneous leishmaniasis. Parasit Vectors. 2018;11:3–11. doi: 10.1186/s13071-018-2908-3. PubMed DOI PMC

Kwakye-Nuako G, Mosore MT, Duplessis C, Bates MD, Puplampu N, Mensah-Attipoe I, et al. First isolation of a new species of Leishmania responsible for human cutaneous leishmaniasis in Ghana and classification in the Leishmania enriettii complex. Int J Parasitol. 2015;45:679–684. doi: 10.1016/j.ijpara.2015.05.001. PubMed DOI

Dougall AM, Alexander B, Holt DC, Harris T, Sultan AH, Bates PA, et al. Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of Leishmania in Australia. Int J Parasitol. 2011;41:571–579. doi: 10.1016/j.ijpara.2010.12.008. PubMed DOI

Seblova V, Sadlova J, Vojtkova B, Votypka J, Carpenter S, Bates PA, et al. The biting midge Culicoides sonorensis (Diptera: Ceratopogonidae) is capable of developing late stage infections of Leishmania enriettii. PLoS Negl Trop Dis. 2015;9:e0004060. doi: 10.1371/journal.pntd.0004060. PubMed DOI PMC

Muniz J, Medina H. Cutaneous leishmaniasis of the guinea pig, Leishmania enriettii n. sp. Hospital (Rio J) 1948;33:7–25. PubMed

Dedet JP, Roche B, Pratlong F, Caies-Quist D, Jouannelle J, Benichou JC, et al. Diffuse cutaneous infection caused by a presumed monoxenous trypanosomatid in a patient infected with HIV. Trans R Soc Trop Med Hyg. 1995;89:644–646. doi: 10.1016/0035-9203(95)90427-1. PubMed DOI

Müller N, Welle M, Lobsiger L, Stoffel MH, Boghenbor KK, Hilbe M, et al. Occurrence of Leishmania sp. in cutaneous lesions of horses in Central Europe. Vet Parasitol. 2009;166:346–351. doi: 10.1016/j.vetpar.2009.09.001. PubMed DOI

Lobsiger L, Müller N, Schweizer T, Frey CF, Wiederkehr D, Zumkehr B, et al. An autochthonous case of cutaneous bovine leishmaniasis in Switzerland. Vet Parasitol. 2010;169:408–414. doi: 10.1016/j.vetpar.2010.01.022. PubMed DOI

Reuss SM, Dunbar MD, Calderwood Mays MB, Owen JL, Mallicote MF, Archer LL, et al. Autochthonous Leishmania siamensis in horse, Florida, USA. Emerg Infect Dis. 2012;18:1545–1547. doi: 10.3201/eid1809.120184. PubMed DOI PMC

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, Siriyasatien P, et al. First isolation of Leishmania from northern Thailand: case report, identification as Leishmania martiniquensis and phylogenetic position within the Leishmania enriettii complex. PLoS Negl Trop Dis. 2014;8:e3339. doi: 10.1371/journal.pntd.0003339. PubMed DOI PMC

Sukmee T, Siripattanapipong S, Mungthin M, Worapong J, Rangsin R, Samung Y, et al. A suspected new species of Leishmania, the causative agent of visceral leishmaniasis in a Thai patient. Int J Parasitol. 2008;38:617–622. doi: 10.1016/j.ijpara.2007.12.003. PubMed DOI

Guimarães VCFV, Pruzinova K, Sadlova J, Volfova V, Myskova J, Filho SPB, et al. Lutzomyia migonei is a permissive vector competent for Leishmania infantum. Parasit Vectors. 2016;9:159. doi: 10.1186/s13071-016-1444-2. PubMed DOI PMC

Liautaud B, Vignier N, Miossec C, Plumelle Y, Kone M, Delta D, et al. First case of visceral leishmaniasis caused by Leishmania martiniquensis. Am J Trop Med Hyg. 2015;92:317–319. doi: 10.4269/ajtmh.14-0205. PubMed DOI PMC

Boisseau-Garsaud AM, Cales-Quist D, Desbois N, Jouannelle J, Jouannelle A, Pratlong F, et al. A new case of cutaneous infection by a presumed monoxenous trypanosomatid in the island of Martinique (French West Indies) Trans R Soc Trop Med Hyg. 2000;94:51–52. doi: 10.1016/S0035-9203(00)90435-8. PubMed DOI

Rose K, Curtis J, Baldwin T, Mathis A, Kumar B, Sakthianandeswaren A, et al. Cutaneous leishmaniasis in red kangaroos: isolation and characterisation of the causative organisms. Int J Parasitol. 2004;34:655–664. doi: 10.1016/j.ijpara.2004.03.001. PubMed DOI

Machado MI, Milder RV, Pacheco RS, Silva M, Braga RR, Lainson R. Naturally acquired infections with Leishmania enriettii Muniz and Medina 1948 in guinea-pigs from São Paulo, Brazil. Parasitology. 1994;109:135–138. doi: 10.1017/S0031182000076241. PubMed DOI

Paraense WL. The spread of Leishmania enriettii through the body of the guinea pig. Trans R Soc Trop Med Hyg. 1953;47:556–560. doi: 10.1016/S0035-9203(53)80008-8. PubMed DOI

Bryceson AD, Bray RS, Wolstencroft RA, Dumonde DC. Cell mediated immunity in cutaneous leishmaniasis of the guinea-pig. Trans R Soc Trop Med Hyg. 1970;64:472. doi: 10.1016/0035-9203(70)90047-7. PubMed DOI

Paranaiba LF, Pinheiro LJ, Macedo DH, Menezes-Neto A, Torrecilhas AC, Tafuri WL, et al. An overview on Leishmania (Mundinia) enriettii: biology, immunopathology, LRV and extracellular vesicles during the host–parasite interaction. Parasitology. 2017;145:1265–1273. doi: 10.1017/S0031182017001810. PubMed DOI

Thomaz-Soccol V, Pratlong F, Langue R, Castro E, Luz E, Dedet JP. New isolation of Leishmania enriettii Muniz and Medina, 1948 in Paraná State, Brazil, 50 years after the first description, and isoenzymatic polymorphism of the L. enriettii taxon. Ann Trop Med Parasitol. 1996;90:491–495. doi: 10.1080/00034983.1996.11813074. PubMed DOI

Belehu A, Turk JL. Establishment of cutaneous Leishmania enriettii infection in hamsters. Infect Immun. 1976;13:1235–1241. doi: 10.1128/IAI.13.4.1235-1241.1976. PubMed DOI PMC

Loría-Cervera EN, Andrade-Narváez FJ. Review: animal models for the study of leishmaniasis immunology. Rev Inst Med Trop Sao Paulo. 2014;56:1–11. doi: 10.1590/S0036-46652014000100001. PubMed DOI PMC

Steppe lemmings and Chinese hamsters as new potential animal models for the study of the Leishmania subgenus Mundinia (Kinetoplastida: Trypanosomatidae)

Experimental transmission of Leishmania (Mundinia) parasites by biting midges (Diptera: Ceratopogonidae)