Leishmania, the dixenous trypanosomatid parasites, are the causative agents of leishmaniasis currently divided into four subgenera: Leishmania, Viannia, Sauroleishmania, and the recently described Mundinia, consisting of six species distributed sporadically all over the world infecting humans and/or animals. These parasites infect various mammalian species and also cause serious human diseases, but their reservoirs are unknown. Thus, adequate laboratory models are needed to enable proper research of Mundinia parasites. In this complex study, we compared experimental infections of five Mundinia species (L. enriettii, L. macropodum, L. chancei, L. orientalis, and four strains of L. martiniquensis) in three rodent species: BALB/c mouse, Chinese hamster (Cricetulus griseus) and steppe lemming (Lagurus lagurus). Culture-derived parasites were inoculated intradermally into the ear pinnae and progress of infection was monitored for 20 weeks, when the tissues and organs of animals were screened for the presence and quantity of Leishmania. Xenodiagnoses with Phlebotomus duboscqi were performed at weeks 5, 10, 15 and 20 post-infection to test the infectiousness of the animals throughout the experiment. BALB/c mice showed no signs of infection and were not infectious to sand flies, while Chinese hamsters and steppe lemmings proved susceptible to all five species of Mundinia tested, showing a wide spectrum of disease signs ranging from asymptomatic to visceral. Mundinia induced significantly higher infection rates in steppe lemmings compared to Chinese hamsters, and consequently steppe lemmings were more infectious to sand flies: In all groups tested, they were infectious from the 5th to the 20th week post infection. In conclusion, we identified two rodent species, Chinese hamster (Cricetulus griseus) and steppe lemming (Lagurus lagurus), as candidates for laboratory models for Mundinia allowing detailed studies of these enigmatic parasites. Furthermore, the long-term survival of all Mundinia species in steppe lemmings and their infectiousness to vectors support the hypothesis that some rodents have the potential to serve as reservoir hosts for Mundinia.

Department of Parasitology Faculty of Science Charles University Prague Czech Republic

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Espinosa OA, Serrano MG, Camargo EP, Teixeira MMG, Shaw JJ. An appraisal of the taxonomy and nomenclature of trypanosomatids presently classified as PubMed

Leishmaniasis [Internet]. [cited 2023 Feb 28]. Available from: https://www.who.int/news-room/fact-sheets/detail/leishmaniasis

Akilov OE, Khachemoune A, Hasan T. Clinical manifestations and classification of Old World cutaneous leishmaniasis. International Journal of Dermatology. 2007, 46(2), 132–142. doi: 10.1111/j.1365-4632.2007.03154.x PubMed DOI

Loría-Cervera EN, Andrade-Narváez FJ. Review: Animal models for the study of leishmaniasis immunology. Rev Inst Med Trop Sao Paulo. 2014; 56(1):1–11. PubMed PMC

Sadlova J, Becvar T, Volf P. Transmission of Enigmatic

Kwakye-Nuako G, Mosore MT, Boakye D, Bates PA. Description, Biology, and Medical Significance of PubMed DOI

Dougall AM, Alexander B, Holt DC, Harris T, Sultan AH, Bates PA, et al. Evidence incriminating midges (Diptera: Ceratopogonidae) as potential vectors of PubMed DOI

Becvar T, Vojtkova B, Siriyasatien P, Votypka J, Modry D, Jahn P, et al. Experimental transmission of PubMed DOI PMC

Seblova V, Sadlova J, Vojtkova B, Votypka J, Carpenter S, Bates PA, et al. The Biting Midge PubMed DOI PMC

Chanmol W, Jariyapan N, Somboon P, Bates MD, Bates PA. Development of PubMed DOI

Muniz J, Medina H. Leishmaniose tegumentar do cobaio, PubMed

Rose K, Curtis J, Baldwin T, Mathis A, Kumar B, Sakthianandeswaren A, et al. Cutaneous leishmaniasis in red kangaroos: Isolation and characterisation of the causative organisms. Int J Parasitol. 2004; 34(6), 655–664. doi: 10.1016/j.ijpara.2004.03.001 PubMed DOI

Dougall A, Shilton C, Low Choy J, Alexander B, Walton S. New reports of Australian cutaneous leishmaniasis in Northern Australian macropods. Epidemiol Infect. 2009; 45(11), 679–684. doi: 10.1017/S0950268809002313 PubMed DOI

Grove SS. Leishmaniasis in South West Africa/Namibia to date. South African Med J. 1989;75(6):290–2. PubMed

Kwakye-Nuako G, Mosore MT, Duplessis C, Bates MD, Puplampu N, Mensah-Attipoe I, et al. First isolation of a new species of PubMed

Bualert L, Charungkiattikul W, Thongsuksai P, Mungthin M, Siripattanapipong S, Khositnithikul R, et al. Case report: Autochthonous disseminated dermal and visceral leishmaniasis in an AIDS patient, Southern Thailand, caused by PubMed PMC

Pothirat T, Tantiworawit A, Chaiwarith R, Jariyapan N, Wannasan A, Siriyasatien P, et al. First Isolation of PubMed DOI PMC

Lobsiger L, Müller N, Schweizer T, Frey CF, Wiederkehr D, Zumkehr B, et al. An autochthonous case of cutaneous bovine leishmaniasis in Switzerland. Vet Parasitol. 2010; doi: 10.1016/j.vetpar.2010.01.022 PubMed DOI

Müller N, Welle M, Lobsiger L, Stoffel MH, Boghenbor KK, Hilbe M, et al. Occurrence of PubMed

Reuss SM, Dunbar MD, Calderwood Mays MB, Owen JL, Mallicote MF, Archer LL, et al. Autochthonous PubMed PMC

Supsrisunjai C, Kootiratrakarn T, Puangpet P, Bunnag T, Chaowalit P, Wessagowit V. Case report: Disseminated autochthonous dermal leishmaniasis caused by PubMed PMC

Jariyapan N, Daroontum T, Jaiwong K, Chanmol W, Intakhan N, Sor-Suwan S, et al. PubMed PMC

Anugulruengkitt S, Songtaweesin WN, Thepnarong N, Tangthanapalakul A, Sitthisan M, Chatproedprai S, et al. Case Report: Simple Nodular Cutaneous Leishmaniasis Caused by Autochthonous PubMed DOI PMC

Manomat J, Leelayoova S, Bualert L, Tan-ariya P, Siripattanapipong S, Mungthin M, et al. Prevalence and risk factors associated with PubMed DOI PMC

Srivarasat S, Brownell N, Siriyasatien P, Noppakun N, Asawanonda P, Rattanakorn K, et al. Case Report: Autochthonous Disseminated Cutaneous, Mucocutaneous, and Visceral Leishmaniasis Caused by PubMed PMC

Kweku MA, Odoom S, Puplampu N, Desewu K, Nuako GK, Gyan B, et al. An outbreak of suspected cutaneous leishmaniasis in Ghana: lessons learnt and preparation for future outbreaks. Global health action. 2011, 4(1), 5527. doi: 10.3402/gha.v4i0.5527 PubMed DOI PMC

Chusri S, Thammapalo S, Silpapojakul K, Siriyasatien P. Animal reservoirs and potential vectors of PubMed

Chaves LF, Hernandez MJ, Dobson AP, Pascual M. Sources and sinks: revisiting the criteria for identifying reservoirs for American cutaneous leishmaniasis. Trends Parasitol. 2007; 23(7), 311–316. doi: 10.1016/j.pt.2007.05.003 PubMed DOI

Smyly HJ, Young CW. The experimental transmission of leishmaniasis to animals. Proc Soc Exp Biol Med. 1924;21:354–6.

Hindle E, Patton WS. Reports from the Royal Society’s Kala Azar Commission in China. No. 2.—Experiments Bearing on the Susceptibility of the Striped Hamster (Cricetulus griseus) to

Young CW, Smyly HJ, Brown C. Experimental kala azar in a hamster,

Meleney HE. The Histopathology of Kala-Azar in the Hamster, Monkey, and Man. Am J Pathol. 1925; 1(2), 147. PubMed PMC

Vojtkova B, Spitzova T, Votypka J, Lestinova T, Kominkova I, Hajkova M, et al. Central Asian rodents as model animals for PubMed DOI PMC

Lun ZR, Wu MS, Chen YF, Wang JY, Zhou XN, Liao LF, et al. Visceral leishmaniasis in China: An endemic disease under Control. Clin Microbiol Rev. 2015; 28(4), 987–1004. doi: 10.1128/CMR.00080-14 PubMed DOI PMC

Sadlova J, Vojtkova B, Lestinova T, Becvar T, Frynta D, Benallal KE, et al. Infectiousness of Asymptomatic PubMed DOI PMC

Sadlova J, Vojtkova B, Becvar T, Lestinova T, Spitzova T, Bates P, et al. Host competence of the African rodents PubMed DOI PMC

Pruzinova K, Sadlova J, Myskova J, Lestinova T, Janda J, Volf P. PubMed PMC

Dostálová A, Volf P. PubMed PMC

Sadlova J, Volf P. Peritrophic matrix of PubMed PMC

Walters LL. PubMed

Čiháková J, Volf P. Development of different PubMed

Sadlova J, Bacikova D, Becvar T, Vojtkova B, England M, Shaw J, et al. PubMed PMC

Belehu A, Turk JL. Establishment of cutaneous PubMed PMC

Paranaiba LF, Pinheiro LJ, Macedo DH, Menezes-Neto A, Torrecilhas AC, Tafuri WL, et al. An overview on PubMed DOI

Thomaz-Soccol V. New isolation of PubMed

Paraense WL. The spread of PubMed

Becvar T, Siriyasatien P, Bates P, Volf P, Sádlová J. Development of PubMed PMC

Intakhan N, Chanmol W, Kongkaew A, Somboon P, Bates MD, Bates PA, et al. Experimental infection of PubMed DOI

Wilson ME, Jeronimo SMB, Pearson RD. Immunopathogenesis of infection with the visceralizing PubMed

Sadlova J, Seblova V, Votypka J, Warburg A, Volf P. Xenodiagnosis of PubMed DOI PMC

Garin YJF, Sulahian A, Méneceur P, Pratlong F, Prina E, Gangneux JP, et al. Experimental pathogenicity of a presumed monoxenous trypanosomatid isolated from humans in a murine model. J Eukaryot Microbiol. 2001; 48(2), 170–176. doi: 10.1111/j.1550-7408.2001.tb00299.x PubMed DOI

Somboonpoonpol N. Parasite burden, distribution and imunopathology of

Falú MA, Fernanda M, Bustos G, María C, Ramoneda P, Raspi EM De, et al. Susceptibility of different mouse strains to

Sriwongpan P, Nedsuwan S, Manomat J, Charoensakulchai S, Lacharojana K, Sankwan J, et al. Prevalence and associated risk factors of leishmania infection among immunocompetent hosts, a community based study in Chiang Rrai, Tthailand. PLoS Negl Trop Dis. 2021; 15(7), e0009545. PubMed PMC

Vojtkova B, Frynta D, Spitzova T, Lestinova T, Votypka J, Volf P, et al. Repeated Sand Fly Bites of Infected BALB/c Mice Enhance the Development of

Valverde JG, Paun A, Inbar E, Romano A, Lewis M, Ghosh K, et al. Increased transmissibility of PubMed PMC

Sadlova J, Vojtkova B, Hrncirova K, Lestinova T, Spitzova T, Becvar T, et al. Host competence of African rodents PubMed PMC