Five lines of four Leishmania major strains, which differ in geographical origin and virulence for mice, were used for experimental infections of Phlebotomus papatasi and P. duboscqi. Differences between the lines, which became evident 6 and 9 days after the infective feed, were more pronounced in P. papatasi. The highest infection rates were found for the more virulent line of strain LV561, while the lowest rates were recorded for strains L119 (low-virulence for mice) and Neal (avirulent for mice). Infection rates depended significantly on the Leishmania strain/line, but not on the vector species. Anterior migration and colonization of the stomodeal valve were observed in flies infected with LV561 and FV1 but infections with other strains were restricted to the whole midgut (L119) or to the abdominal midgut only (Neal). The proportions of the different morphological forms of Leishmania seen in gut smears of infected flies varied considerably with the parasite strain/line. In general, vector forms of LV561 and FV1 were characterized by relatively long flagella and bodies. The strains developing less successfully in vectors tended to have a relatively broad body (L119) or short flagellum (Neal). Transmission experiments were successful with P. duboscqi females infected with the virulent line of LV561.

Department of Parasitology Charles University Prague 2 Czech Republic

Citace poskytuje Crossref.org

The development of L. major, L. donovani and L. martiniquensis, Leishmania currently emerging in Europe, in the sand fly species Phlebotomus perniciosus and P. tobbi

Discovery of essential kinetoplastid-insect adhesion proteins and their function in Leishmania-sand fly interactions

Steppe lemmings and Chinese hamsters as new potential animal models for the study of the Leishmania subgenus Mundinia (Kinetoplastida: Trypanosomatidae)

Characterization of a new Leishmania major strain for use in a controlled human infection model

Lower galactosylation levels of the Lipophosphoglycan from Leishmania (Leishmania) major-like strains affect interaction with Phlebotomus papatasi and Lutzomyia longipalpis

Leishmania HASP and SHERP Genes Are Required for In Vivo Differentiation, Parasite Transmission and Virulence Attenuation in the Host

Lutzomyia migonei is a permissive vector competent for Leishmania infantum

The Biting Midge Culicoides sonorensis (Diptera: Ceratopogonidae) Is Capable of Developing Late Stage Infections of Leishmania enriettii

Transmission potential of antimony-resistant leishmania field isolates

The effect of avian blood on Leishmania development in Phlebotomus duboscqi

Leishmania development in sand flies: parasite-vector interactions overview

The development of Leishmania turanica in sand flies and competition with L. major

Experimental transmission of Leishmania infantum by two major vectors: a comparison between a viscerotropic and a dermotropic strain

Visualisation of Leishmania donovani fluorescent hybrids during early stage development in the sand fly vector

The stage-regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi

Leishmania major glycosylation mutants require phosphoglycans (lpg2-) but not lipophosphoglycan (lpg1-) for survival in permissive sand fly vectors

Peritrophic matrix of Phlebotomus duboscqi and its kinetics during Leishmania major development

Increased transmission potential of Leishmania major/Leishmania infantum hybrids