With only three published reports, the genus Chroogomphus (Boletales, Gomphidiaceae) is poorly studied in Pakistan. During recent sampling events in Khyber Pakhtunkhawa province, Pakistan, several collections of Chroogomphus were made, representing undescribed taxa. Based on morphological and molecular data, two new species are described: Chroogomphus pakistanicus and C. pruinosus. We present a description and illustrations for both taxa. A molecular phylogenetic reconstruction, based on the internal transcribed spacer (ITS1-5.8S-ITS2) barcode region, shows that C. pakistanicus and C. pruinosus are placed in two different subgenera of Chroogomphus (subg. Chroogomphus and subg. Siccigomphus, respectively).

Department of Botany and Plant Pathology Purdue University USA

Department of Botany University of the Punjab Quaid e Azam Campus Lahore 54590 Pakistan

Faculty of Science University of South Bohemia Czech Republic

Zobrazit více v PubMed

Accioly T, Sousa JO, Moreau P-A, Lécuru C, Silva BDB, Roy M, Gardes M, Baseia IG, Martín MP. (2019) Hidden fungal diversity from the Neotropics: PubMed DOI PMC

Agerer R. (1990) Studies on ectomycorrhizae XXIV. Ectomycorrhizae of DOI

Agerer R. (1991) Studies on ectomycorrhizae XXXIV. Mycorrhizae of

Ahmad S, Iqbal SH, Khalid AN. (1997) Fungi of Pakistan. Sultan Ahmad Mycological Society of Pakistan, Department of Botany, University of the Punjab, Quaid-e-Azam campus, Lahore, Pakistan.

Aime MC. (2004) Intercompatibility tests and phylogenetic analysis in the

Breitenbach J, Kränzlin F. (1991) Pilze der Schweiz. Band 3 Röhrling und Blätterpilze 1. Teil. Switzerland: Verlag Mykologia, 353 pp.

Chernomor O, Von Haeseler A, Minh BQ. (2016) Terrace aware data structure for phylogenomic inference from supermatrices. Systematic Biology 65: 997–1008. 10.1093/sysbio/syw037 PubMed DOI PMC

Dai YC, Tolgor B. (2007) Illustration of Edible and Medicinal Fungi in Northeastern China. Beijing: Science Press, 231 pp. [in Chinese]

Edgar RC. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. 10.1093/nar/gkh340 PubMed DOI PMC

Fries EM. (1836) Corpus Florarum provincialium suecicae I. Floram Scanicam. 349 pp. 10.5962/bhl.title.47083 DOI

Gardes M, Bruns TD. (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. 10.1111/j.1365-294X.1993.tb00005.x PubMed DOI

Gerhardt E. (1984) Pilze. Band 1: Lamellenpilze, Täublinge, Milchlinge und andere Gruppen mit Lamellen. München: BLV Verlagsgesellschaft, 319 pp.

Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hoang DT, Chernomor O, Von Haeseler A, Minh BQ, Vinh LS. (2017) UFBoot2: improving the ultrafast bootstrap approximation. Molecular Biology and Evolution 35: 518–22. 10.1093/molbev/msx281 PubMed DOI PMC

Horak E. (2005) Röhrlinge und Blätterpilze in Europa. Munich: Spektrum Akademischer Verlag, 557 pp.

Jumbam B, Haelewaters D, Koch RA, Dentinger BTM, Henkel TW, Aime MC. (2019) A new and unusual species of DOI

Kalyaanamoorthy K, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. 10.1038/nmeth.4285 PubMed DOI PMC

Knudsen H, Taylor A. (2012)

Legon NW, Henrici A. (2005) Checklist of the British and Irish Basidiomycota. Kew: Royal Botanic Gardens.

Li YC, Yang ZL, Tolgor B. (2009) Phylogenetic and biogeographic relationships of

Martín MP, Siquier JL, Salom JC, Telleria MT, Finschow G. (2016) Barcoding sequences clearly separate DOI

Miller OK. (1964) Monograph of Chroogomphus (Gomphidiaceae). Mycologia 56: 526–549. 10.1080/00275514.1964.12018141 DOI

Miller OK. (2003) The PubMed DOI

Miller OK, Aime MC. (2001) Systematics, ecology and world distribution in the genus Chroogomphus (Gomphidiaceae). In: Misra JK, Horn BW. (Eds) Trichomycetes and other fungal groups.Science Publishers, Enfield, New Hampshire, 315–333.

Munsell Soil Color Charts (1975) Munsell Color Co., Baltimore, Maryland, USA.

Nguyen L-T, Schmidt HA, Von Haeseler A, Minh BQ. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution 32: 268–274. 10.1093/molbev/msu300 PubMed DOI PMC

Raithelhuber J. (1974) Hongos de la Provincial de Buenos Aires y de la capital federal. Buenos Aires: J. Raithelhuber.

Razaq A, Ilyas S, Khalid AN. (2016) Molecular identification of Chinese DOI

Scambler R, Niskanen T, Assyov B, Ainsworth AM, Bellanger JM, Loizides M, Moreau PA, Kirk PM, Liimatainen K. (2018) Diversity of PubMed PMC

Shinwari ZK, Rehman M, Watanabe T, Yoshikawa Y. (2006) Medicinal and aromatic plants of Pakistan. A Pictorial Guide. Kohat University of Science and Technology, Pakistan.

Siddiqui MF, Shaukat SS, Ahmed N, Khan NA, Khan IA. (2013) Vegetation-Environment relationship of conifer dominating forests of moist temperate belt of Himalayan and Hindukush regions of Pakistan. Pakistan Journal of Botany 452: 577–592.

Singer R. (1949) The genus DOI

Singer R, Kuthan J. (1976) Notes on

Singh G, Dal Grande F, Divakar PK, Otte J, Leavitt SD, Szczepanska K, Crespo A, Rico VJ, Aptroot A, da Silva Cáceres ME, Lumbsch HT, Schmitt I. (2015) Coalescent-based species delimitation approach uncovers high cryptic diversity in the cosmopolitan lichen-forming fungal genus PubMed DOI PMC

Sochorová Z, Döbbeler P, Sochor M, van Rooy J. (2019) PubMed DOI PMC

Villarreal M, Heykoop M. (1996)

Wahab M. (2011) Population dynamics and dendrochronological potential of pine tree species of District Dir Pakistan. Ph.D. dissertation. Department of Botany, Federal Urdu University, Karachi, Pakistan.

White TJ, Bruns TD, Lee SB, Taylor JW. (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ. (Eds) PCR Protocols: a guide to methods and applications.Academic Press, San Diego, 315–322. 10.1016/B978-0-12-372180-8.50042-1 DOI

Xie ZX, Wang Y, Wang B. (1986) Illustration of agarics of Changbai Mountains, China. Jilin Scientific and Technology Press, Changchun, 1–288. [in Chinese]

Yu FQ, Liu PG. (2005) Species diversity of wild edible mushrooms from DOI

New species of Pseudosperma (Agaricales, Inocybaceae) from Pakistan revealed by morphology and multi-locus phylogenetic reconstruction