Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR
Language English Country Italy Media print-electronic
Document type Journal Article
PubMed
32310299
PII: 496N149
Knihovny.cz E-resources
- Keywords
- Streptococcus pneumoniae, community-acquired pneumonia, qPCR, quantification, specificity,
- MeSH
- DNA, Bacterial genetics MeSH
- Respiratory System microbiology MeSH
- Community-Acquired Infections * diagnosis microbiology MeSH
- Real-Time Polymerase Chain Reaction * MeSH
- Humans MeSH
- Pneumonia, Pneumococcal * diagnosis microbiology MeSH
- Sensitivity and Specificity MeSH
- Streptococcus pneumoniae genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with community-acquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, based on lytA, determined a cutoff value of ~2x104, 4x102 and 4x102 DNA copies per 1 mL of valid sputum, tracheal aspirate and bronchial aspirate samples, respectively. The results from the present study suggest that qPCR detection of all three genes would be optimal in the accurate detection of Streptococcus pneumoniae.
