The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with community-acquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, based on lytA, determined a cutoff value of ~2x104, 4x102 and 4x102 DNA copies per 1 mL of valid sputum, tracheal aspirate and bronchial aspirate samples, respectively. The results from the present study suggest that qPCR detection of all three genes would be optimal in the accurate detection of Streptococcus pneumoniae.

• MeSH
• DNA bakterií genetika   MeSH
• dýchací soustava mikrobiologie   MeSH
• infekce získané v komunitě * diagnóza   mikrobiologie   MeSH
• kvantitativní polymerázová řetězová reakce * MeSH
• lidé MeSH
• pneumonie pneumokoková * diagnóza   mikrobiologie   MeSH
• senzitivita a specificita MeSH
• Streptococcus pneumoniae genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Controversies still exist regarding definition of the thrombotic risks in Ph- (BCR/ABL1-) myeloproliferative disorders with thrombocythemia (MPD-T). Platelet counts at diagnosis are currently not taken as a risk factor of thrombosis. In our cohort of 1179 patients with MPD-T, prospectively registered for anagrelide treatment, we found that the median platelet count prior to the thrombotic event was significantly higher than at time points without any ensuing thrombosis (453 vs. 400 × 10(9)/L, P < 0.001), albeit higher platelet counts at diagnosis tended to be connected with fewer thrombotic events (in contrast to WBC counts at diagnosis). The JAK2(V617F) mutation predicted both arterial and venous events, while age >65 yr, hypertension, diabetes mellitus, smoking, elevated triglyceride and homocysteine levels predicted arterial events only. For venous events, the specific thrombophilic risk factors (factor V 'Leiden' and others), antiphospholipid antibodies, and elevated factor VIII levels played a major role. During anagrelide treatment (± aspirin), we documented a decrease in both venous (6.7-fold) and arterial events (1.8-fold), while bleeding (mostly minor events) increased twofold compared to history. Our results suggest that keeping platelet counts at low levels may be a meaningful therapeutic measure to prevent thrombosis, although their counts at diagnosis lack any prognostic value.

• MeSH
• Aspirin aplikace a dávkování   MeSH
• bcr-abl fúzové proteiny MeSH
• chinazoliny aplikace a dávkování   MeSH
• filadelfský chromozom * MeSH
• Janus kinasa 2 genetika   MeSH
• lidé MeSH
• missense mutace MeSH
• počet trombocytů MeSH
• prospektivní studie MeSH
• registrace * MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• substituce aminokyselin MeSH
• trombocytóza * krev   komplikace   farmakoterapie   genetika   MeSH
• trombóza * krev   farmakoterapie   etiologie   genetika   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH