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Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR
R. Kukla, R. Bolehovska, J. Radocha, L. Pliskova, P. Zak, F. Vrbacky, J. Nekvindova, H. Zemlickova,
Jazyk angličtina Země Itálie
Typ dokumentu časopisecké články
Grantová podpora
NV17-28539A
MZ0
CEP - Centrální evidence projektů
PubMed
32310299
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií genetika MeSH
- dýchací soustava mikrobiologie MeSH
- infekce získané v komunitě * diagnóza mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce * MeSH
- lidé MeSH
- pneumonie pneumokoková * diagnóza mikrobiologie MeSH
- senzitivita a specificita MeSH
- Streptococcus pneumoniae genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with community-acquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, based on lytA, determined a cutoff value of ~2x104, 4x102 and 4x102 DNA copies per 1 mL of valid sputum, tracheal aspirate and bronchial aspirate samples, respectively. The results from the present study suggest that qPCR detection of all three genes would be optimal in the accurate detection of Streptococcus pneumoniae.
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- $a Kukla, Rudolf $u Department of Clinical Microbiology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Czech Republic.
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- $a Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR / $c R. Kukla, R. Bolehovska, J. Radocha, L. Pliskova, P. Zak, F. Vrbacky, J. Nekvindova, H. Zemlickova,
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- $a The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with community-acquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, based on lytA, determined a cutoff value of ~2x104, 4x102 and 4x102 DNA copies per 1 mL of valid sputum, tracheal aspirate and bronchial aspirate samples, respectively. The results from the present study suggest that qPCR detection of all three genes would be optimal in the accurate detection of Streptococcus pneumoniae.
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- $a Bolehovska, Radka $u Department of Clinical Microbiology, University Hospital Hradec Kralove, Czech Republic.
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- $a Radocha, Jakub $u 4th Department of Internal Medicine - Hematology, Faculty Hospital, Charles University, Hradec Kralove, Czech Republic.
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- $a Pliskova, Lenka $u Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic.
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- $a Zak, Pavel $u 4th Department of Internal Medicine - Hematology, Faculty Hospital, Charles University, Hradec Kralove, Czech Republic.
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- $a Vrbacky, Filip $u 4th Department of Internal Medicine - Hematology, Faculty Hospital, Charles University, Hradec Kralove, Czech Republic.
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- $a Nekvindova, Jana $u Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Czech Republic.
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- $a Zemlickova, Helena $u Department of Clinical Microbiology, University Hospital Hradec Kralove, Faculty of Medicine in Hradec Kralove, Charles University, Czech Republic. National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic.
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- $w MED00005605 $t The new microbiologica $x 1121-7138 $g Roč. 43, č. 2 (2020), s. 70-77
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