Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Adult worm, Digoxigenin labeling, Fluorescence RNA detection, In situ hybridization, RNA probe, Schistosoma mansoni, Tissue section,
- MeSH
- digoxigenin metabolismus MeSH
- hybridizace in situ fluorescenční metody MeSH
- messenger RNA genetika metabolismus MeSH
- RNA sondy metabolismus MeSH
- Schistosoma mansoni cytologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- digoxigenin MeSH
- messenger RNA MeSH
- RNA sondy MeSH
In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Prague Czech Republic
Institute of Specific Prophylaxis and Tropical Medicine Medical University of Vienna Vienna Austria
Citace poskytuje Crossref.org
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