Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Adult worm, Digoxigenin labeling, Fluorescence RNA detection, In situ hybridization, RNA probe, Schistosoma mansoni, Tissue section,
- MeSH
- Digoxigenin metabolism MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- RNA, Messenger genetics metabolism MeSH
- RNA Probes metabolism MeSH
- Schistosoma mansoni cytology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Digoxigenin MeSH
- RNA, Messenger MeSH
- RNA Probes MeSH
In situ hybridization is a tool for evaluation of gene expression within tissues or single cells. This protocol describes optimized sensitive fluorescence detection of gene transcripts (mRNAs) in semithin sections of Schistosoma mansoni adult worms using specifically designed and labeled RNA probes. Due to improved methodologies in tissue preservation, sectioning, amplification of fluorescent signal, and prehybridization tissue treatment, it is possible to detect transcripts in the fine structures of schistosomes. The protocol is sensitive enough to detect very low abundance targets. This procedure is optimized for tissues derived from S. mansoni adult worms; however, it can be successfully applied to other trematode species.
Department of Parasitology Faculty of Science Charles University Prague Czech Republic
Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences Prague Czech Republic
Institute of Specific Prophylaxis and Tropical Medicine Medical University of Vienna Vienna Austria
References provided by Crossref.org
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