Auxin biosynthesis in the phytopathogenic fungus Leptosphaeria maculans is associated with enhanced transcription of indole-3-pyruvate decarboxylase LmIPDC2 and tryptophan aminotransferase LmTAM1
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
32540203
DOI
10.1016/j.resmic.2020.05.001
PII: S0923-2508(20)30054-1
Knihovny.cz E-zdroje
- Klíčová slova
- Dothideomycetes, Hormone, Indole-3-pyruvate decarboxylase, Tryptamine, Tryptophan, Tryptophan aminotransferase,
- MeSH
- aminohydrolasy genetika MeSH
- biosyntetické dráhy MeSH
- Brassica napus mikrobiologie MeSH
- fylogeneze MeSH
- genetická transkripce MeSH
- houby klasifikace genetika metabolismus MeSH
- karboxylyasy genetika metabolismus MeSH
- kyseliny indoloctové metabolismus farmakologie MeSH
- Leptosphaeria enzymologie genetika růst a vývoj metabolismus MeSH
- regulace genové exprese u hub MeSH
- regulátory růstu rostlin metabolismus MeSH
- tryptaminy metabolismus farmakologie MeSH
- tryptofan metabolismus farmakologie MeSH
- tryptofantransaminasa genetika metabolismus MeSH
- upregulace MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aminohydrolasy MeSH
- indoleacetic acid MeSH Prohlížeč
- indolepyruvate decarboxylase MeSH Prohlížeč
- karboxylyasy MeSH
- kyseliny indoloctové MeSH
- nitrilase MeSH Prohlížeč
- regulátory růstu rostlin MeSH
- tryptamine MeSH Prohlížeč
- tryptaminy MeSH
- tryptofan MeSH
- tryptofantransaminasa MeSH
Auxins are hormones that regulate growth and development in plants. Besides plants, various microorganisms also produce auxins. Here we investigate whether and how the phytopathogenic fungus Leptosphaeria maculans biosynthesizes auxins. We characterized the auxin profile of in vitro grown L. maculans. The culture was further supplied with the auxin biosynthetic-precursors tryptophan and tryptamine and gene expression and phytohormone content was analyzed. L. maculans in vitro produced IAA (indole-3-acetic acid) as the predominant auxin metabolite. IAA production could be further stimulated by supplying precursors. Expression of indole-3-pyruvate decarboxylase LmIPDC2, tryptophan aminotransferase LmTAM1 and nitrilase LmNIT1 genes was mainly upregulated after adding tryptophan and correlated with IAA production, suggesting that these genes are the key components of auxin biosynthesis in L. maculans. Tryptamine acted as a potent inducer of IAA production, though a pathway independent of LmIPDC2/LmTAM1 may be involved. Despite L. maculans being a rich source of bioactive IAA, the auxin metabolic profile of host plant Brassica napus was not altered upon infection. Exogenous IAA inhibited the growth of L. maculans in vitro when supplied in high concentration. Altogether, we showed that L. maculans is capable of IAA production and we have identified biosynthetic genes that were responsive to tryptophan treatment.
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