The Russula globispora lineage is a morphologically and phylogenetically well-defined group of ectomycorrhizal fungi occurring in various climatic areas. In this study we performed a multi-locus phylogenetic study based on collections from boreal, alpine and arctic habitats of Europe and Western North America, subalpine collections from the southeast Himalayas and collections from subtropical coniferous forests of Pakistan. European and North American collections are nearly identical and probably represent a single species named R. dryadicola distributed from the Alps to the Rocky Mountains. Collections from the southeast Himalayas belong to two distinct species: R. abbottabadensis sp. nov. from subtropical monodominant forests of Pinus roxburghii and R. tengii sp. nov. from subalpine mixed forests of Abies and Betula. The results suggest that speciation in this group is driven by a climate disjunction and adaptation rather than a host switch and geographical distance.

Department of Botany Faculty of Natural Sciences Comenius University in Bratislava Révová 39 SK 811 02 Bratislava Slovakia

Department of Botany University of the Punjab Quaid e Azam Campus Lahore 54590 Pakistan

Department of Cryptogams Institute of Botany Plant Science and Biodiversity Centre Slovak Academy of Sciences Dúbravská cesta 9 SK 845 23 Bratislava Slovakia

Department of Plant Sciences Quaid i Azam University Islamabad 45320 Pakistan

Farlow Reference Library and Herbarium of Cryptogamic Botany Harvard University Cambridge MA 02138 USA

Laboratoire IMPECS Fac Pharma Lille Université de Lille F 59000 Lille France

Laboratory of Fungal Genetics and Metabolism Institute of Microbiology Czech Academy of Sciences Vídeňská 1083 CZ 142 20 Praha Czech Republic

State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences No 3 1st Beichen West Road Chaoyang District Beijing 100101 China

Zobrazit více v PubMed

ADAMČÍK SLAVOMÍR, CABOŇ MIROSLAV, EBERHARDT URSULA, SABA MALKA, HAMPE FELIX, SLOVÁK MAREK, KLEINE JESKO, MARXMÜLLER HELGA, JANČOVIČOVÁ SOŇA, PFISTER DONALD H., KHALID ABDUL N., KOLAŘÍK MIROSLAV, MARHOLD KAROL, VERBEKEN ANNEMIEKE. A molecular analysis reveals hidden species diversity within the current concept of Russula maculata (Russulaceae, Basidiomycota) Phytotaxa. 2016;270(2):71. doi: 10.11646/phytotaxa.270.2.1. DOI

Adamčík S, Jančovičová S. Type studies in Russula subsection Maculatinae and affiliated taxa: four species as interpreted by Henri Romagnesi. Sydowia. 2013;65:201–222.

Adamčík S, Marhold K. Taxonomy of the Russula xerampelina group. I. Morphometric study of the Russula xerampelina group in Slovakia. Mycotaxon. 2000;76:463–479.

Adamčík S, Slovák M, Eberhardt U, Ronikier A, Jairus T, et al. Molecular inference, multivariate morphometrics and ecological assessment are applied in concert to delimit species in the Russula clavipes complex. Mycologia. 2016;108:716–730. doi: 10.3852/15-194. PubMed DOI

Bahram M, Põlme S, Koljalg U, Zarre S, Tedersoo L. Regional and local patterns of ectomycorrhizal fungal diversity and community structure along an altitudinal gradient in the Hyrcanian forests of northern Iran. New Phytologist. 2012;193:465–473. doi: 10.1111/j.1469-8137.2011.03927.x. PubMed DOI

Bazzicalupo AL, Buyck B, Saar I, Vauras J, Carmean D, Berbee ML. Troubles with mycorrhizal mushroom identification where morphological differentiation lags behind barcode sequence divergence. Taxon. 2017;66:791–810. doi: 10.12705/664.1. DOI

Biscoletto A, Ostellari C (2005) Russula dryadicola (Singer) Fellner & Landa, primo ritrovamento in Italia. Rivista di Mycologia 4:301–307

Buyck B (1989) Valeur taxonomique du bleu de crésyl pour le genre Russula. Bulletin Trimestriel de la Societé Mycologique de France 105:1–6

Buyck B. The study of microscopic features in Russula 2. Sterile cells of the hymenium. Russulales News. 1991;1:62–85.

Caboň M, Eberhardt U, Looney BP, Hampe F, Kolařík M, et al. New insights in Russula subsect. Rubrinae: phylogeny and the quest for synapomorphic characters. Mycological Progress. 2017;16:877–892. doi: 10.1007/s11557-017-1322-0. DOI

Cao Y, Zhang Y, Yu Z, Mi F, Liu C, et al. Structure, gene flow, and recombination among geographic populations of a Russula virescens ally from southwestern China. PLoS One. 2013;8:e73174. doi: 10.1371/journal.pone.0073174. PubMed DOI PMC

Das K, Dowie NJ, Li GJ, Miller SL. Two new species of Russula (Russulales) from India. Mycosphere. 2014;5:612–622. doi: 10.5943/mycosphere/5/5/2. DOI

Das K, Ghosh A, Bhatt RP, Chakraborty D, Hofstetter V, Buyck B (2017a) Fungal biodiversity profiles 41–50. Cryptogamie, Mycologie 38:527–547

Das K, Ghosh A, Chakraborty DF, Li J, Qiu L et al (2017b) Fungal biodiversity profiles 31–40. Cryptogamie, Mycologie. Mycologie 38:353–406

Donoghue MJ. A phylogenetic perspective on the distribution of plant diversity. Proceedings of the National Academy of Sciences, USA. 2008;105(Suppl. 1):11549–11555. doi: 10.1073/pnas.0801962105. PubMed DOI PMC

Eberhardt U. Methods for DNA barcoding of fungi. In: Kress WJ, Erickson DL, editors. DNA barcodes methods and protocols. New York: Humana Press; 2012. pp. 183–205. PubMed

Fellner R, Landa J. Some species of Cortinariaceae and Russulaceae in the alpine belt of the Belaer Tatras. Bibliotheca Mycologica. 1993;150:33–37.

Feng G, Mi X, Eiserhardt WL, Jin G, Sang W, et al. Assembly of forest communities across East Asia insights from phylogenetic community structure and species pool scaling. Scientific Reports. 2015;5:9337. doi: 10.1038/srep09337. PubMed DOI PMC

Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Molecular Ecology. 1993;2:113–118. doi: 10.1111/j.1365-294X.1993.tb00005.x. PubMed DOI

Geml J, Timling I, Robinson CH, Lennon N, Nusbaum HC, et al. An arctic community of symbiotic fungi assembled by long-distance dispersers: phylogenetic diversity of ectomycorrhizal basidiomycetes in Svalbard based on soil and sporocarp DNA. Journal of Biogeography. 2012;39:74–88. doi: 10.1111/j.1365-2699.2011.02588.x. DOI

Gouy M, Guindon S, Gascuel O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Molecular Biology and Evolution. 2010;27:221–224. doi: 10.1093/molbev/msp259. PubMed DOI

Hongo T. The Agaricales of Japan I-3. Russulaceae. Acta Phytotaxonomica Geobotanica. 1960;18:129–146.

Joshi S, Bhatt RP, Stephenson SL. The current status of the family Russulaceae in the Uttarakhand Himalaya, India. Mycosphere. 2012;3:486–501. doi: 10.5943/mycosphere/3/4/12. DOI

Katoh K, Standley DM. MAFFT multiple sequence alignment software, version 7: improvements in performance and usability. Molecular Biology and Evolution. 2013;30:772–780. doi: 10.1093/molbev/mst010. PubMed DOI PMC

Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, et al. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012;28:1647–1649. doi: 10.1093/bioinformatics/bts199. PubMed DOI PMC

Knudsen H, Borgen T. New and rare taxa of Russula from Greenland. Persoonia. 1992;14:509–517.

Knudsen H, Borgen T (1992b) Russulaceae in Greenland. In: Arctic and Alpine Mycology (Laursen GA, Ammirati JF, eds) 1: 216–244. Seattle: University of Washington Press

Kühner R (1975) Agaricales de la zone Alpine. Genre Russula Pers, ex S. F. Gray. Bulletin Trimestriel de la Societé Mycologique de France 91:313–390

Lanfear R, Calcott B, Ho SY, Guindon S, Lanfear R, et al. PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution. 2012;29:1695–1701. doi: 10.1093/molbev/mss020. PubMed DOI

Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, et al. Fungal diversity notes 253–366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity. 2016;78:1–237. doi: 10.1007/s13225-016-0366-9. DOI

Li GJ, Li SF, Liu XZ, Wen HA. Russula jilinensis sp. nov. (Russulaceae) from Northeast China. Mycotaxon. 2012;120:49–58. doi: 10.5248/120.49. DOI

Looney BP, Meidl P, Piatek MJ, Miettinen O, Martin FM, et al. Russulaceae: a new genomic dataset to study ecosystem function and evolutionary diversification of ectomycorrhizal fungi with their tree associates. New Phytologist. 2018;218:54–65. doi: 10.1111/nph.15001. PubMed DOI

Looney BP, Ryberg M, Hampe F, Sánchez-García M, Matheny PB. Into and out of the tropics: global diversification patterns in a hyperdiverse clade of ectomycorrhizal fungi. Molecular Ecology. 2016;25:630–647. doi: 10.1111/mec.13506. PubMed DOI

Marstad P. Russula in the Nordic countries. Tønsberg: P Marstad. 2004.

Matheny PB. Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales) Molecular Phylogenetics and Evolution. 2005;35:1–20. doi: 10.1016/j.ympev.2004.11.014. PubMed DOI

Miehe G, Miehe S, Böhner J, Bäumler R, Ghimire SK, et al. Vegetation ecology. In: Miehe G, Pendry CA, Chaudhary R, et al., editors. An Introduction to the natural history, ecology and human environment of the Himalayas Nepal. Edinburgh: Royal Botanic Garden; 2015. pp. 385–472.

Põlme S, Bahram M, Yamanaka T, Nara K, Dai YC, et al. Biogeography of ectomycorrhizal fungi associated with alders (Alnus spp.) in relation to biotic and abiotic variables at the global scale. New Phytologist. 2013;198:1239–1249. doi: 10.1111/nph.12170. PubMed DOI

Rambaut A, Suchard MA, Xie D, Drummond AJ (2013) Tracer. Version 1.6. http://beast.bio.ed.ac.uk/software/tracer/

Romagnesi H. Les Russules d’Europe et d'Afrique du Nord. Paris: Bordas; 1967.

Ronquist F, Teslenko M, van der Mark P, Avres DL, Darling A, et al. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice, across a large model space. Systematic Biology. 2012;61:539–542. doi: 10.1093/sysbio/sys029. PubMed DOI PMC

Ruotsalainen J, Vauras J. Novelties in Russula : R. olivobrunnea, R. intermedia and R. groenlandica. Karstenia. 1994;34:21–34. doi: 10.29203/ka.1994.303. DOI

Ryberg M. Molecular operational taxonomic units as approximations of species in the light of evolutionary models and empirical data from Fungi. Molecular Ecology. 2015;24:5770–5777. doi: 10.1111/mec.13444. PubMed DOI

Sarnari M. Monographia Illustrata del Genere Russula in Europa. Trento: Associazione Micologica Bresadola; 1998.

Silvestro Daniele, Michalak Ingo. raxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution. 2011;12(4):335–337. doi: 10.1007/s13127-011-0056-0. DOI

Singer R. Zur Russula-Forschung. Zeitschrift für Pilzkunde. 1925;5:73–80.

Singer R. Monographie der Gattung Russula. Hedwigia. 1926;66:153–260.

Singer R (1938) Contribution a l’étude des Russules (1). 3. Quelques Russules américaines et asiatiques. Bulletin de la Société Mycologique de France Fr 54:132–177

Stamatakis A (2008) The RAxML 7.0.4 manual. https://web.natur.cuni.cz/~vlada/moltax/RAxML-Manual.7.0.4.pdf

Tian H, Liu T, Lian J, Li G, Ba T. Identification and classification of four Russula species from Inner Mongolia based on morphology and ITS sequencing. Acta Edulis Fungi. 2014;4:646.

White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: a guide to methods and applications. San Diego: Academic Press; 1990. pp. 315–322.

Taxonomic reintroduction of Taphrinaviridis (Taphrinales, Ascomycota) associated with Alnusalnobetula as one of five well defined European species colonizing alders

Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex

Fungal Planet description sheets: 951-1041