In vitro biological evaluation and consideration about structure-activity relationship of silver(I) aminoacidate complexes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32652262
DOI
10.1016/j.jinorgbio.2020.111170
PII: S0162-0134(20)30198-7
Knihovny.cz E-resources
- Keywords
- Anticancer activity, Antimicrobial activity, Groove binding, Nuclease activity, Silver(I) complexes, Stability in solution,
- MeSH
- Alanine chemistry metabolism pharmacology MeSH
- Anti-Bacterial Agents chemistry metabolism pharmacology MeSH
- Antifungal Agents chemistry metabolism pharmacology MeSH
- Candida parapsilosis drug effects MeSH
- DNA metabolism MeSH
- Escherichia coli drug effects MeSH
- Phenylalanine chemistry metabolism pharmacology MeSH
- Catalysis MeSH
- Coordination Complexes chemistry metabolism pharmacology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Molecular Structure MeSH
- Cell Line, Tumor MeSH
- Cell Proliferation drug effects MeSH
- Antineoplastic Agents chemistry metabolism pharmacology MeSH
- Drug Screening Assays, Antitumor MeSH
- Cattle MeSH
- Staphylococcus aureus drug effects MeSH
- DNA Cleavage drug effects MeSH
- Silver chemistry MeSH
- Structure-Activity Relationship MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alanine MeSH
- Anti-Bacterial Agents MeSH
- Antifungal Agents MeSH
- calf thymus DNA MeSH Browser
- DNA MeSH
- Phenylalanine MeSH
- Coordination Complexes MeSH
- Antineoplastic Agents MeSH
- Silver MeSH
Two silver(I) aminoacidate complexes {[Ag4(L-HAla)4(NO3)3]NO3}n (AgAla, complex 1, Ala = alanine) and {[Ag(L-Phe)]}n (AgPhe, complex 2, Phe = phenylalanine) were prepared and characterized by elemental, spectral analysis (FT-IR, NMR techniques) and single crystal X-ray analysis in solid state and their solution stability was measured in biological testing time-scale by 1H NMR. The bridging coordination modes of the zwitterionic Ala and deprotonated Phe ligands led to the formation of 1D polymeric chains of the complexes. The significant argentophilic interactions are presented in the structure of AgAla. Antimicrobial testing of prepared Ag(I) complexes was evaluated by IC50 and MIC values and were compared with AgGly, silver(I) sulfadiazine and AgNO3 samples. Moreover, MTS test was used to the testing of broad range antiproliferative activity of studied compounds against different cancer cell lines and also to the investigation of calf thymus DNA interactions by absorption spectroscopy, fluorescence spectroscopy, Ethidium bromide/Hoechst 33258 displacement experiments and circular dichroism spectroscopy. To evaluate the pUC19 DNA fragmentation by silver(I) complexes, the agarose gel electrophoresis was used. In addition to biological evaluation we used lipophilicity measurement results in the discussion about structure-activity relationship (SAR).
References provided by Crossref.org
Antimicrobial and Anticancer Application of Silver(I) Dipeptide Complexes
