Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
32996917
DOI
10.1039/d0an01667e
Knihovny.cz E-resources
- MeSH
- beta-Galactosidase genetics MeSH
- Fluorescent Dyes * MeSH
- Galactose MeSH
- Microfluidics * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- beta-Galactosidase MeSH
- Fluorescent Dyes * MeSH
- Galactose MeSH
We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-d-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.
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