Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu časopisecké články
PubMed
32996917
DOI
10.1039/d0an01667e
Knihovny.cz E-zdroje
- MeSH
- beta-galaktosidasa genetika MeSH
- fluorescenční barviva * MeSH
- galaktosa MeSH
- mikrofluidika * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- beta-galaktosidasa MeSH
- fluorescenční barviva * MeSH
- galaktosa MeSH
We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-d-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode.
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