Immune responses in mice vaccinated with a DNA vaccine expressing a new elastase from Trichinella spiralis
Language English Country Czech Republic Media electronic
Document type Journal Article
PubMed
33082302
DOI
10.14411/fp.2020.027
PII: 2020.027
Knihovny.cz E-resources
- Keywords
- Trichinella, protective immunity, trichinellosis, vaccination,
- MeSH
- Vaccines, DNA administration & dosage MeSH
- Mice MeSH
- Pancreatic Elastase administration & dosage immunology pharmacology MeSH
- Helminth Proteins administration & dosage immunology pharmacology MeSH
- Trichinella spiralis immunology MeSH
- Trichinellosis immunology parasitology MeSH
- Vaccination * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Vaccines, DNA MeSH
- Pancreatic Elastase MeSH
- Helminth Proteins MeSH
The elastase, which belongs to the serine protease family, hydrolyses various proteins and may be involved in the parasite invasion. In this study, complete sequence of elastase-1 (TsE) the nematode Trichinella spiralis (Owen, 1835) was cloned into the plasmid pcDNA3.1 as TsE DNA vaccine. After intramuscular vaccination, serum anti-Trichinella antibodies (IgG and subclass IgG1/IgG2a, and IgA), total and specific intestinal mucosal sIgA in mice vaccinated with pcDNA3.1/TsE were measured by ELISA. The results showed that vaccination with pcDNA3.1/TsE induced a systemic humoral immune response (high levels of serum IgG and subclass IgG1/IgG2a and IgA) and local intestinal mucosal immune responses (high levels of TsE-specific sIgA). Vaccination of mice with TsE DNA vaccine also triggered a systemic and local concomitant Th1/Th2 response, as demonstrated by significant elevation of Th1 (IFN-γ and IL-2) / Th2 (IL-4 and IL-10) cytokine levels after the spleen, mesenteric lymph node and Peyer's patch cells from vaccinated mice were stimulated with recombinant TsE (rTsE). The vaccination of mice with pcDNA3.1/TsE displayed a 17% reduction of intestinal adult worms and a 39% reduction of muscle larvae. Our results indicated that TsE DNA vaccine elicited a systemic concomitant Th1/Th2 response and an enteral local sIgA response, and produced a partial protection against infection with T. spiralis. The TsE may be regarded as a potential candidate vaccine target against Trichinella infection. The oral polyvalent vaccines should be developed to improve the protective efficacy of anti-Trichinella vaccines.
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