Utility of NKX3.1 immunohistochemistry in the differential diagnosis of seminal vesicles versus prostatic tissue in needle biopsy
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
33126153
DOI
10.1016/j.anndiagpath.2020.151644
PII: S1092-9134(20)30190-8
Knihovny.cz E-zdroje
- Klíčová slova
- Immunohistochemistry, NKX3.1, Prostate, Prostatic adenocarcinoma, Seminal vesicles,
- MeSH
- adenokarcinom diagnóza MeSH
- diferenciální diagnóza MeSH
- homeodoménové proteiny analýza metabolismus MeSH
- imunohistochemie MeSH
- jehlová biopsie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery analýza metabolismus MeSH
- nádory prostaty diagnóza MeSH
- prostata metabolismus MeSH
- semenné váčky metabolismus patologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- transkripční faktory analýza metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- homeodoménové proteiny MeSH
- nádorové biomarkery MeSH
- NKX3-1 protein, human MeSH Prohlížeč
- transkripční faktory MeSH
NKX3.1 is considered a reliable immunohistochemical marker of prostatic origin with high specificity and sensitivity. However, NKX3.1 positivity has been described in other neoplastic and non-neoplastic tissues, such as mesenchymal chondrosarcoma, sex-cord stromal tumors, rete testis adenocarcinoma, lobular and ductal carcinoma of the breast, salivary glands, peribronchial submucosal glands, and Sertoli cells. We analyzed expression of two antibodies (mono and polyclonal) of NKX3.1 in a total of 63 non-neoplastic seminal vesicles. We used 52 resection materials (12 seminal vesicles without prostatic adenocarcinoma, 26 seminal vesicles with prostatic adenocarcinoma infiltration, and 14 cases of seminal vesicles infiltrated by urothelial carcinoma) and 11 prostatic core needle biopsies with incidentally sampled fragment of seminal vesicles. In all cases, tissues from seminal vesicles were completely negative for NKX3.1, despite using polyclonal and monoclonal NKX3.1 antibodies, and regardless of the detection system utilized (diaminobenzidine (DAB) versus alkaline phosphatase (AF)). However, prostatic adenocarcinoma was negative in several cases (n = 6), when AF detection system was used. Reaction with DAB was strong and robust in all cases. Based on our data, we can recommend NKX3.1 as a negative immunohistochemical marker of seminal vesicles.
Department of Pathology Charles University Prague Faculty of Medicine in Plzeň Pilsen Czech Republic
Department of Urology Charles University Prague Faculty of Medicine in Plzeň Pilsen Czech Republic
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