Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, multicentrická studie, práce podpořená grantem
PubMed
33161592
PubMed Central
PMC8247412
DOI
10.1111/bjh.17210
Knihovny.cz E-zdroje
- Klíčová slova
- ALL, AML, acute leukaemia, flow cytometry, immunophenotyping, myeloperoxidase,
- MeSH
- akutní nemoc MeSH
- imunofenotypizace normy MeSH
- leukemie * diagnóza enzymologie imunologie MeSH
- lidé MeSH
- nádorové proteiny * krev imunologie MeSH
- peroxidasa * krev imunologie MeSH
- průtoková cytometrie normy MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- MPO protein, human MeSH Prohlížeč
- nádorové proteiny * MeSH
- peroxidasa * MeSH
Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.
Cancer Research Center Salamanca Spain
Centro de Investigaciόn Biomédicaen Red de Cáncer Instituto Carlos 3 Madrid Spain
Department of Hematology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands
Department of Immunology Leiden University Medical Center Leiden The Netherlands
Department of Pediatric Hematology and Oncology Zabrze Medical University of Silesia Katowice Poland
Dutch Childhood Oncology Group Utrecht the Netherlands
Institute for Laboratory Medicine Kantonsspital Aarau AG Aarau Switzerland
Princess Máxima Center for Pediatric Oncology Utrecht the Netherlands
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