Screening of novel feedstuffs, that too for data-deficient (nutritionally) animals, is somewhat ambiguous or problematic. Through systematic meta-analyses, the present study formulated most up-to-date crayfish nutritional standards, against which a recyclable waste (biofloc biomass, BM) from intensive aquaculture systems was assessed as a novel protein source. Growth trajectory dependencies and thermal growth coefficient qualifying for good growth in crayfish (TGC 0.5-0.64 units) were benchmarked. Using these standards and a 7-week growth trial, BM's suitability as a novel protein source for red swamp crayfish Procambarus clarkii was evaluated through its graded inclusions in a commercial feed. Results suggest that BM can elevate growth at 33-66% inclusion in existing feed formulations. Beyond 66% inclusion, BM can deteriorate growth in crayfish due to high ash content (exceeding physiological limit > 14%), arginine deficiency (~ 14-20% lower than an optimum requirement), and insufficient non-protein energy: protein ratio (3.7 cal mg-1). Arginine is perhaps the most critical amino acid in dietary protein for crayfish, and deficient in BM. Although no critical bioaccumulation levels of heavy metals were breached by feeding 100% BM to crayfish, a mineral and heavy metal (Hg) stress seemed plausible. Crayfish raised solely on biofloc may not realize full growth potential.

Faculty of Fisheries and Protection of Waters South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses University of South Bohemia in České Budějovice Na Sádkách 1780 370 05 Ceske Budejovice Czech Republic

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