Killer yeasts are attractive antifungal agents with great potential applications in the food industry. Natural Saccharomyces paradoxus isolates provide new dsRNA-based killer systems available for investigation. The presence of viral dsRNA may alter transcriptional profile of S. paradoxus. To test this possibility, a high-throughput RNA sequencing was employed to compare the transcriptomes of S. paradoxus AML 15-66 K66 killer strains after curing them of either M-66 alone or both M-66 and L-A-66 dsRNA viruses. The S. paradoxus cells cured of viral dsRNA(s) showed respiration deficient or altered sporulation patterns. We have identified numerous changes in the transcription profile of genes including those linked to ribosomes and amino acid biosynthesis, as well as mitochondrial function. Our work advance studies of transcriptional adaptations of Saccharomyces spp. induced by changes in phenotype and set of dsRNA viruses, reported for the first time.

Department of Biochemistry and Molecular Biology Institute of Biosciences Vilnius University Saulėtekio al 7 10257 Vilnius Lithuania

Department of Chemistry and Bioengineering Vilnius Gediminas Technical University Saulėtekio al 11 10223 Vilnius Lithuania

Laboratory of Genetics Institute of Botany Nature Research Centre Akademijos str 2 08412 Vilnius Lithuania

Life Science Research Centre Faculty of Science University of Ostrava Chittussiho 10 710 00 Ostrava Czech Republic

Martsinovsky Institute of Medical Parasitology Tropical and Vector Borne Diseases Sechenov University Malaya Pirogovskaya str 20 119435 Moscow Russia

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