Prostate cancer has the highest malignancy rate diagnosed in men worldwide. Albeit, the gold standard serum prostate-specific antigen (PSA) assays reduced the mortality rate of the disease, the number of false positive diagnoses steeply increased. Therefore, there is an urgent need for complementary biomarkers to enhance the specificity and selectivity of current diagnostic methods. Information about PSA glycosylation can help to fulfill this gap as alterations of its carbohydrate moieties due to cancerous transformation may represent additional markers to distinguish malignant from benign tumors. However, development of suitable methods and instrumentations to investigate the N-glycosylation profile of PSA represents a challenge. In this paper, we critically review the current bioanalytical trends and strategies in the field of PSA glycobiomarker research focusing on separation based characterization methods.

Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic Brno Czech Republic

Translational Glycomics Research Group Research Institute of Biomolecular and Chemical Engineering Faculty of Engineering University of Pannonia Veszprem Hungary

Translational Glycomics Research Group Research Institute of Biomolecular and Chemical Engineering Faculty of Engineering University of Pannonia Veszprem Hungary; Horváth Csaba Memorial Laboratory of Bioseparation Sciences Research Centre for Molecular Medicine Doctoral School of Molecular Medicine Faculty of Medicine University of Debrecen Debrecen Hungary

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