Molecular patterns of isolated tubulitis differ from tubulitis with interstitial inflammation in early indication biopsies of kidney allografts
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33335257
PubMed Central
PMC7746707
DOI
10.1038/s41598-020-79332-9
PII: 10.1038/s41598-020-79332-9
Knihovny.cz E-resources
- MeSH
- Allografts metabolism pathology MeSH
- Biomarkers * MeSH
- Biopsy MeSH
- Nephritis, Interstitial diagnosis etiology MeSH
- Humans MeSH
- Graft Survival genetics immunology MeSH
- Graft Rejection etiology metabolism pathology MeSH
- Gene Expression Profiling MeSH
- Transcriptome MeSH
- Kidney Transplantation * adverse effects MeSH
- Computational Biology MeSH
- High-Throughput Nucleotide Sequencing MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers * MeSH
The Banff 2019 kidney allograft pathology update excluded isolated tubulitis without interstitial inflammation (ISO-T) from the category of borderline (suspicious) for acute T cell-mediated rejection due to its proposed benign clinical outcome. In this study, we explored the molecular assessment of ISO-T. ISO-T or interstitial inflammation with tubulitis (I + T) was diagnosed in indication biopsies within the first 14 postoperative days. The molecular phenotype of ISO-T was compared to I + T either by using RNA sequencing (n = 16) or by Molecular Microscope Diagnostic System (MMDx, n = 51). RNA sequencing showed lower expression of genes related to interferon-y (p = 1.5 *10-16), cytokine signaling (p = 2.1 *10-20) and inflammatory response (p = 1.0*10-13) in the ISO-T group than in I + T group. Transcripts with increased expression in the I + T group overlapped significantly with previously described pathogenesis-based transcript sets associated with cytotoxic and effector T cell transcripts, and with T cell-mediated rejection (TCMR). MMDx classified 25/32 (78%) ISO-T biopsies and 12/19 (63%) I + T biopsies as no-rejection. ISO-T had significantly lower MMDx scores for interstitial inflammation (p = 0.014), tubulitis (p = 0.035) and TCMR (p = 0.016) compared to I + T. Fewer molecular signals of inflammation in isolated tubulitis suggest that this is also a benign phenotype on a molecular level.
Alberta Transplant Applied Genomics Centre Edmonton AB T6G 2S2 Canada
Transplant Laboratory Institute for Clinical and Experimental Medicine 140 21 Prague Czech Republic
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