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PubMed

Záznam pochází z PubMed

Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas

Hulstaert, Eva
Autor Hulstaert, Eva Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; Department of Dermatology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Morlion, Annelien
Autor Morlion, Annelien Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Avila Cobos, Francisco
Autor Avila Cobos, Francisco Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Verniers, Kimberly
Autor Verniers, Kimberly Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Nuytens, Justine
Autor Nuytens, Justine Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Vanden Eynde, Eveline
Autor Vanden Eynde, Eveline Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Yigit, Nurten
Autor Yigit, Nurten Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Anckaert, Jasper
Autor Anckaert, Jasper Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Geerts, Anja
Autor Geerts, Anja Department of Gastroenterology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Hindryckx, Pieter
Autor Hindryckx, Pieter Department of Gastroenterology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Jacques, Peggy
Autor Jacques, Peggy Department of Rheumatology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium; VIB Inflammation Research Center, Ghent University, Technologiepark 71, 9052 Ghent, Belgium
Brusselle, Guy
Autor Brusselle, Guy Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Bracke, Ken R
Autor Bracke, Ken R Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Maes, Tania
Autor Maes, Tania Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Malfait, Thomas
Autor Malfait, Thomas Department of Respiratory Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Derveaux, Thierry
Autor Derveaux, Thierry Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Ninclaus, Virginie
Autor Ninclaus, Virginie Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Van Cauwenbergh, Caroline
Autor Van Cauwenbergh, Caroline Department of Ophthalmology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Roelens, Kristien
Autor Roelens, Kristien Department of Obstetrics, Women's Clinic, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Roets, Ellen
Autor Roets, Ellen Department of Obstetrics, Women's Clinic, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Hemelsoet, Dimitri
Autor Hemelsoet, Dimitri Department of Neurology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Tilleman, Kelly
Autor Tilleman, Kelly Department of Reproductive Medicine, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Brochez, Lieve
Autor Brochez, Lieve OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; Department of Dermatology, Ghent University Hospital, C. Heymanslaan 10, 9000 Ghent, Belgium
Kuersten, Scott
Autor Kuersten, Scott Illumina, San Diego, CA, USA
Simon, Lukas M
Autor Simon, Lukas M University of Texas Health Science Center, School of Biomedical Informatics, Center for Precision Health, Houston, TX, USA
Karg, Sebastian
Autor Karg, Sebastian Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Germany
Kautzky-Willers, Alexandra
Autor Kautzky-Willers, Alexandra Department of Internal Medicine III, Clinical Division of Endocrinology and Metabolism, Unit of Gender Medicine, Medical University of Vienna, Vienna, Austria
Leutner, Michael
Autor Leutner, Michael Department of Internal Medicine III, Clinical Division of Endocrinology and Metabolism, Unit of Gender Medicine, Medical University of Vienna, Vienna, Austria
Nöhammer, Christa
Autor Nöhammer, Christa Austrian Institute of Technology, Center for Health and Bioresources, Molecular Diagnostics, Vienna, Austria
Slaby, Ondrej
Autor Slaby, Ondrej Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic; Department of Pathology, University Hospital Brno, Brno, Czech Republic; Central European Institute of Technology, Masaryk University, Brno, Czech Republic
Prins, Roméo Willinge
Autor Prins, Roméo Willinge Department of Oncogenomics, Amsterdam University Medical Centers (AUMC), University of Amsterdam, Amsterdam, the Netherlands
Koster, Jan
Autor Koster, Jan Department of Oncogenomics, Amsterdam University Medical Centers (AUMC), University of Amsterdam, Amsterdam, the Netherlands
Lefever, Steve
Autor Lefever, Steve Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Schroth, Gary P
Autor Schroth, Gary P Illumina, San Diego, CA, USA
Vandesompele, Jo
Autor Vandesompele, Jo Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium
Mestdagh, Pieter
Autor Mestdagh, Pieter Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium; OncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, C. Heymanslaan 10, 9000 Ghent, Belgium. Electronic address: pieter.mestdagh@ugent.be

Cell reports. 2020 Dec 29 ; 33 (13) : 108552.

Cell Rep
ISSN 2211-1247
Zdroj

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz https://www.medvik.cz/link/pmid33378673

PubMed 33378673
DOI 10.1016/j.celrep.2020.108552
PII: S2211-1247(20)31541-2
Knihovny.cz E-zdroje

Klíčová slova
RNA sequencing, biofluids, biomarker, cell-free RNA, circular RNA, extracellular RNA, liquid biopsy, messenger RNA, small RNA,
MeSH
biologické markery * MeSH
kohortové studie MeSH
lidé MeSH
RNA genetika metabolismus MeSH
sekvenční analýza RNA metody MeSH
stanovení celkové genové exprese metody MeSH
tělesné tekutiny metabolismus MeSH
transkriptom * MeSH
Check Tag
lidé MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Research Support, N.I.H., Extramural MeSH
Názvy látek
biologické markery * MeSH
RNA MeSH

Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other biofluids may be more informative. We present an atlas of messenger, circular, and small RNA transcriptomes of a comprehensive collection of 20 human biofluids. By means of synthetic spike-in controls, we compare RNA content across biofluids, revealing a 10,000-fold difference in concentration. The circular RNA fraction is increased in most biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. Our atlas enables an informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Spike-normalized data are publicly available in the R2 web portal for further exploration.

Austrian Institute of Technology Center for Health and Bioresources Molecular Diagnostics Vienna Austria

Center for Medical Genetics Department of Biomolecular Medicine Ghent University C Heymanslaan 10 9000 Ghent Belgium; OncoRNALab Cancer Research Institute Ghent Ghent University C Heymanslaan 10 9000 Ghent Belgium

Department of Gastroenterology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Internal Medicine 3 Clinical Division of Endocrinology and Metabolism Unit of Gender Medicine Medical University of Vienna Vienna Austria

Department of Neurology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Obstetrics Women's Clinic Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Oncogenomics Amsterdam University Medical Centers University of Amsterdam Amsterdam the Netherlands

Department of Ophthalmology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Reproductive Medicine Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Respiratory Medicine Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium

Department of Rheumatology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium; VIB Inflammation Research Center Ghent University Technologiepark 71 9052 Ghent Belgium

Helmholtz Zentrum München German Research Center for Environmental Health Institute of Computational Biology Neuherberg Germany