The structure and function of soil archaea across biomes
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33582288
DOI
10.1016/j.jprot.2021.104147
PII: S1874-3919(21)00046-4
Knihovny.cz E-resources
- Keywords
- 16S rRNA gene amplicon sequencing, Archaea, Climate, Metaproteomics, Soil, Vegetation,
- MeSH
- Archaea * genetics MeSH
- Ecosystem MeSH
- Soil * MeSH
- Soil Microbiology MeSH
- RNA, Ribosomal, 16S MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Soil * MeSH
- RNA, Ribosomal, 16S MeSH
We lack a predictive understanding of the environmental drivers determining the structure and function of archaeal communities as well as the proteome associated with these important soil organisms. Here, we characterized the structure (by 16S rRNA gene sequencing) and function (by metaproteomics) of archaea from 32 soil samples across terrestrial ecosystems with contrasting climate and vegetation types. Our multi-"omics" approach unveiled that genes from Nitrosophaerales and Thermoplasmata dominated soils collected from four continents, and that archaea comprise 2.3 ± 0.3% of microbial proteins in these soils. Aridity positively correlated with the proportion of Nitrosophaerales genes and the number of archaeal proteins. The interaction of climate x vegetation shaped the functional profile of the archaeal community. Our study provides novel insights into the structure and function of soil archaea across climates, and highlights that these communities may be influenced by increasing global aridity.
CEBAS CSIC Campus Universitario de Espinardo Murcia E 30100 Spain
Departamento de Sistemas Físicos Químicos y Naturales Universidad Pablo de Olavide Sevilla Spain
Otto von Guericke University Bioprocess Engineering Universitätsplatz 2 39106 Magdeburg Germany
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