The effect of two porphyrine photosensitizers TMPyP and ZnTPPS4 for application in photodynamic therapy of cancer cells in vitro
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
33609757
DOI
10.1016/j.pdpdt.2021.102224
PII: S1572-1000(21)00050-8
Knihovny.cz E-zdroje
- Klíčová slova
- Cancer cells, PDT, Photodynamic therapy, Porphyrine photosensitizer, TMPyP, ZnTPPS(4),
- MeSH
- fotochemoterapie * metody MeSH
- fotosenzibilizující látky farmakologie terapeutické užití MeSH
- metaloporfyriny * MeSH
- nádory * farmakoterapie MeSH
- porfyriny * farmakologie MeSH
- reaktivní formy kyslíku MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fotosenzibilizující látky MeSH
- metaloporfyriny * MeSH
- porfyriny * MeSH
- reaktivní formy kyslíku MeSH
- zinc-tetrakis(p-sulfonatophenyl)porphyrin MeSH Prohlížeč
Photodynamic therapy (PDT) is one of the treatments for cancer. This therapy uses a combination of a photosensitizer (PS), light irradiation, and oxygen O2, which is converted to cytotoxic 1O2 and other forms of reactive oxygen species (ROS), causing selective damage to the target tissue. In this work, we studied effect of two porphyrin photosensitizers TMPyP and ZnTPPS4 at three different concentrations (0.25, 0.5, 5μM) after two irradiation doses (5 and 25 J/cm2). Photodynamic efect of TMPyP and ZnTPPS4 were confirmed by a battery of in vitro tests including MTT, reactive oxygen species (ROS) production and mitochondrial membrane potential test (MMP). Morphological changes of the cells before and after treatment were imaged by atomic force microscopy (AFM). The most effective combination of irradiation dose and concentration for both PSs showed a concentration of 5 μM and a irradiation dose of 25 J/cm2 in both cell cultures.
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