Fructosyltransferase (FTase) catalyzes the transfer of a fructosyl group to a sucrose molecule or a fructooligosaccharide (FOS) when a FOS with a longer chain is formed. Production of FTase by two Aspergillus species and its mixture was exploited using solid-state fermentation (SSF) and employing agave sap as substrate. The maximum FTase activity (1.59 U/mL) by Aspergillus oryzae was obtained after 24 h, using a temperature of 30 °C, with an inoculum of 2 × 107 spores/mL. The nucleotide sequence coding for the fructosyltransferase showed 1494 bp and encodes for a protein of 498 amino acids. The hypothetical molecular tertiary structure of Aspergillus oryzae BM-DIA FTase showed the presence of structural domains, such as a five-bladed beta-propeller domain characteristic of GH (glycoside hydrolase) and C terminal, which forms a beta-sandwich module. This study contributes to the knowledge of stability, compatibility, and genetic expression of Aspergillus oryzae BM-DIA under SSF bioprocess conditions for industrial production of fructosyltransferase.

Departament de Genética Facultat de Biología Universitat de Barcelona Avenida Diagonal 643 08028 Barcelona Spain

Department of Food Research School of Chemistry Autonomous University of Coahuila Blvd 5 Carranza e Ing José Cárdenas S N República Oriente 25280 Saltillo Coahuila Mexico

Engineering Department Instituto Tecnológico de Ciudad Valles Tecnológico Nacional de México Carr al Ingenio Plan de Ayala Km 2 Col Vista Hermosa 79010 Ciudad Valles San Luis Potosí México

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