BACKGROUND: The endothelial glycocalyx (EG) plays a vital role in the physiology and pathophysiology of human microcirculation. Having relevant EG damage model would be important tool for testing new interventions aiming at EG protection and recovery. We describe the first in vivo EG damage model in pig. OBJECTIVE: To investigate the course of animal EG damage induced by specific enzymes. MATERIAL AND METHODS: Four anesthetized piglets received enzymes: 1g hyaluronidase and 25 IU heparanase I intravenously. Blood and urine samples were collected at baseline and 20/40/60/80/100/120 min for detecting markers of endothelial and EG function. Sublingual microcirculation and EG thickness were assessed by Side-stream Dark Field (SDF) imaging and Perfused Boundary Region (PBR) respectively. EG of the mesentery artery was visualized in fluorescent microscopy. RESULTS: Biochemical marker of EG damage syndecan-1 showed temporary increase with return to baseline and was reflected by PBR values. Albumin levels suggested brief period of capillary leakage (decrease in the serum, increase in the urine) with a trend to normalization. Urine glycosaminoglycans peaked at 120 minutes. Microcirculatory perfusion parameter showed significant alteration. Diffusion parameters were altered with no statistical significance. CONCLUSION: EG damage induced by specific enzymes was reflected by temporary changes of biochemical makers together with alteration of microcirculation and changes in fluorescent microscopy of EG layer. Our results support to further validate presented model of EG damage on a larger number of animals.

Centrum for Research and Development University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Anesthesia Pain Management and Perioperative Medicine Dalhousie University Halifax NS Canada

Department of Anesthesiology Perioperative Medicine and Intensive Care J E Purkinje University Masaryk Hospital Usti nad Labem Czech Republic

Department of Anesthesiology Resuscitation and Intensive Care Medicine University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Clinical Biochemistry and Diagnostics University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Histology and Embryology Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic

Department of Surgery University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Urology University Hospital Hradec Kralove Hradec Kralove Czech Republic

Faculty of Engineering Dept of Electronics and Informatics Vrije Universiteit Brussel Brussels Belgium

Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic

Faculty of Military Health Sciences University of Defense Brno Brno Czech Republic

International Fluid Academy Lovenjoel Belgium

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