Progress in forensic bone DNA analysis: Lessons learned from ancient DNA
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
34265517
DOI
10.1016/j.fsigen.2021.102538
PII: S1872-4973(21)00076-4
Knihovny.cz E-resources
- Keywords
- Ancient DNA, Degraded DNA, Forensic, Inhibitors,
- MeSH
- DNA Fingerprinting MeSH
- DNA * genetics MeSH
- Humans MeSH
- DNA Degradation, Necrotic MeSH
- Aged MeSH
- Forensic Genetics MeSH
- DNA, Ancient * MeSH
- Check Tag
- Humans MeSH
- Aged MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- DNA * MeSH
- DNA, Ancient * MeSH
Research on ancient and forensic DNA is related in many ways, and the two fields must deal with similar obstacles. Therefore, communication between these two communities has the potential to improve results in both research fields. Here, we present the insights gained in the ancient DNA community with regard to analyzing DNA from aged skeletal material and the potential use of the developed protocols in forensic work. We discuss the various steps, from choosing samples for DNA extraction to deciding between classical PCR amplification and massively parallel sequencing approaches. Based on the progress made in ancient DNA analyses combined with the requirements of forensic work, we suggest that there is substantial potential for incorporating ancient DNA approaches into forensic protocols, a process that has already begun to a considerable extent. However, taking full advantage of the experiences gained from ancient DNA work will require comparative studies by the forensic DNA community to tailor the methods developed for ancient samples to the specific needs of forensic studies and case work. If successful, in our view, the benefits for both communities would be considerable.
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