Improving reproducibility and replicability in preclinical research is a widely discussed and pertinent topic, especially regarding ethical responsibility in animal research. INFRAFRONTIER, the European Research Infrastructure for the generation, phenotyping, archiving, and distribution of model mammalian genomes, is addressing this issue by developing internal quality principles for its different service areas, that provides a quality framework for its operational activities. This article introduces the INFRAFRONTIER Quality Principles in Systemic Phenotyping of genetically altered mouse models. A total of 11 key principles are included, ranging from general requirements for compliance with guidelines on animal testing, to the need for well-trained personnel and more specific standards such as the exchange of reference lines. Recently established requirements such as the provision of FAIR (Findable, Accessible, Interoperable, Reusable) data are also addressed. For each quality principle, we have outlined the specific context, requirements, further recommendations, and key references.

Biomedical Sciences Research Center Alexander Fleming Athens Greece

Centre d'Immunophénomique Aix Marseille Université INSERM CNRS CELPHEDIA PHENOMIN Marseille France

Czech Centre for Phenogenomics Institute of Molecular Genetics of the Czech Academy of Sciences Vestec Czech Republic

German Center for Diabetes Research Neuherberg Germany

Helmholtz Zentrum München Institute of Experimental Genetics German Mouse Clinic Neuherberg Germany

INFRAFRONTIER GmbH Neuherberg Germany

Lunenfeld Tanenbaum Research Institute Sinai Health Toronto ON Canada

MRC Harwell Institute Mammalian Genetics Unit Medical Research Council Harwell Campus Oxfordshire UK

MRC Harwell Institute Mary Lyon Centre Harwell Campus Oxfordshire UK

Technische Universität München Chair of Experimental Genetics TUM School of Life Sciences Freising Germany

The Centre for Phenogenomics Toronto ON Canada

The Faculty of Medicine University of Toronto Toronto ON Canada

The Hospital for Sick Children Toronto ON Canada

Universite de Strasbourg CNRS INSERM CELPHEDIA PHENOMIN Institut Clinique de La Souris Illkirch France

University of Oulu and Oulu University Hospital PEDEGO Research Unit and Medical Research Center Oulu Oulu Finland

University of Oulu Biocenter Oulu Transgenic and Tissue Phenotyping Core Facility Oulu Finland

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