A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.

Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelu 27 783 71 Olomouc Czech Republic

Crop Research Institute Drnovská 507 161 06 Praha Czech Republic

Faculty of Biotechnology and Food Science Slovak University of Agriculture Tr A Hlinku 2 949 76 Nitra Slovakia

National Agriculture and Food Centre Research Institute of Plant Production Bratislavská Cesta 122 921 01 Piešťany Slovakia

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