The developments of green-based metallic nanoparticles (gold) are gaining tremendous interest, having potential applications in health care and diagnosis. Therefore, in the present study, Polianthes tuberosa flower filtered extract was used as a reducing and stabilizing agent to synthesize gold nanoparticles (PtubAuNPs). The PtubAuNPs were extensively characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and X-ray diffraction. The antibacterial activity of PtubAuNPs was determined by the agar well diffusion method; the PtubAuNPs performed extreme antagonistic activity against the tested pathogens. Furthermore, the cytotoxicity of the PtubAuNPs was evaluated in MCF 7 cells by MTT assay. The PtubAuNPs induced toxicity in MCF 7 cells with the least concentration of 100 µg/mL in a dose-dependent method by inducing apoptosis. Overall, the study manifested that PtubAuNPs are a potent nanomaterial that can be employed as an antimicrobial and anticancer agent.

Biology Department Science and Humanities College Shaqra University Alquwayiyah 19245 Saudi Arabia

Department of Biotechnology Bharathidasan University Tiruchirappalli 620024 India

Department of Biotechnology Karpagam Academy of Higher Education Coimbatore 641021 India

Department of Chemistry Faculty of Science University of Hradec Kralove 50003 Hradec Kralove Czech Republic

Laboratory of Functional Molecules and Materials School of Physics and Optoelectronic Engineering Shandong University of Technology Zibo 255000 China

Malaysia Japan International Institute of Technology Universiti Teknologi Malaysia Kuala Lumpur 54100 Malaysia

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