Fast Leaps between Millisecond Confinements Govern Ase1 Diffusion along Microtubules
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
LL1602
Ministry of Education, Youth and Sports of the Czech Republic
PubMed
34927934
DOI
10.1002/smtd.202100370
Knihovny.cz E-zdroje
- Klíčová slova
- Ase1, coarse-grain model, energy landscape, interferometric scattering microscopy, scattering labels,
- MeSH
- buněčné dělení MeSH
- časoprostorová analýza MeSH
- mikrotubuly metabolismus MeSH
- prasata MeSH
- proteinové domény MeSH
- proteiny asociované s mikrotubuly chemie metabolismus MeSH
- simulace molekulární dynamiky MeSH
- transport proteinů MeSH
- zobrazení jednotlivé molekuly MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- proteiny asociované s mikrotubuly MeSH
Diffusion is the most fundamental mode of protein translocation within cells. Confined diffusion of proteins along the electrostatic potential constituted by the surface of microtubules, although modeled meticulously in molecular dynamics simulations, has not been experimentally observed in real-time. Here, interferometric scattering microscopy is used to directly visualize the movement of the microtubule-associated protein Ase1 along the microtubule surface at nanometer and microsecond resolution. Millisecond confinements of Ase1 and fast leaps between these positions of dwelling preferentially occurring along the microtubule protofilaments are resolved, revealing Ase1's mode of diffusive translocation along the microtubule's periodic surface. The derived interaction potential closely matches the tubulin-dimer periodicity and the distribution of the electrostatic potential on the microtubule lattice. It is anticipated that mapping the interaction landscapes for different proteins on microtubules, finding plausible energetic barriers of different positioning and heights, can provide valuable insights into regulating the dynamics of essential cytoskeletal processes, such as intracellular cargo trafficking, cell division, and morphogenesis, all of which rely on diffusive translocation of proteins along microtubules.
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