Serum albumin as a primary non-covalent binding protein for nitro-oleic acid
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
35063491
DOI
10.1016/j.ijbiomac.2022.01.050
PII: S0141-8130(22)00060-5
Knihovny.cz E-zdroje
- Klíčová slova
- Adduction, Nitro-fatty acid, Non-covalent interaction, Oleic acid, Reduced albumin, Serum albumin,
- MeSH
- dusíkaté sloučeniny MeSH
- kyselina olejová MeSH
- kyseliny olejové MeSH
- lidé MeSH
- sérový albumin * chemie MeSH
- tandemová hmotnostní spektrometrie MeSH
- transportní proteiny * metabolismus MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- CXA-10 MeSH Prohlížeč
- dusíkaté sloučeniny MeSH
- kyselina olejová MeSH
- kyseliny olejové MeSH
- sérový albumin * MeSH
- transportní proteiny * MeSH
This work explores the interaction of 9/10-nitro-oleic acid (NO2-OA) with human serum albumin (HSA). The molecular mechanism of the biological action of NO2-OA is to our knowledge based on a reversible covalent reaction-Michael addition of nucleophilic amino acid residues of proteins. Since HSA is an important fatty acid transporter, a key question is whether NO2-OA can bind covalently or non-covalently to HSA, similarly to oleic acid (OA), which can interact with the FA1-FA7 binding sites of the HSA molecule. 1H NMR studies and competition analysis with OA and the drugs ibuprofen and warfarin were used to investigate a potential non-covalent binding mode. NO2-OA/HSA binding was confirmed to compete with warfarin for FA-7 with significantly higher affinity. NO2-OA competes with ibuprofen for FA-3 and FA-6, however, in contrast to the situation with warfarin, the binding affinities are not significantly different. The described interactions are based exclusively on non-covalent binding. No covalent binding of NO2-OA to HSA was detected by MS/MS. More detailed studies based on MALDI-TOF-MS and Ellman's assay indicated that HSA can be covalently modified in the presence of NO2-OA to a very limited extent. It was also shown that NO2-OA has a higher affinity to HSA than that of OA.
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