Chronopotentiometric sensing of native, oligomeric, denatured and aggregated serum albumin at charged surfaces
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
35334293
DOI
10.1016/j.bioelechem.2022.108100
PII: S1567-5394(22)00051-2
Knihovny.cz E-zdroje
- Klíčová slova
- Charged surface, Constant current chronopotentiometry, Electrode, Freezing, Scanning transmission electron microscopy, Serum albumin,
- MeSH
- adsorpce MeSH
- denaturace proteinů MeSH
- peptidy MeSH
- sérový albumin hovězí * chemie MeSH
- sérový albumin * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- peptidy MeSH
- sérový albumin hovězí * MeSH
- sérový albumin * MeSH
In protein analysis, fast techniques applicable for preliminary tests of the protein structural changes are sought. We show that using constant current chronopotentiometric stripping peak H, small amounts of oligomeric, denatured and aggregated bovine serum albumin (BSA) can be easily distinguished from native form. Different behavior of native, denatured, and aggregated BSA could be explained by combination of their different adsorption at charged surface and accessibility of electroactive amino acid residues. Ability to discriminate between individual forms allows to use chronopotentiometric stripping for study of processes responsible for structural changes, such as freezing treatment.
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