The study aims to follow up on the analysis of Pressure injuries (PIs) prevalence conducted between 2007 and 2014 and after the new methodological requirements for PIs surveillance establishment at the national level. A retrospective, nationwide cross-sectional analysis of data regarding the STROBE checklist was collected by the National Health Information System (NHIS). The International Classification of Diseases (ICD-10) diagnoses L89.0-L89.9 for PIs were used in the period 2010-2019. A total of 264 442 records of patients with diagnoses of L89.0-L89.9 were identified from 2010 to 2019 (26 444 patients per year on average). The numbers are increasing every year, and there is a 40% increase between 2010 and 2019. When comparing recorded PIs, the percentage of PIs occurrence in category I decreased, and the number of PIs in category IV increased in the second analysed period. Still, in absolute numbers, there is an increase across all categories. The age of patients with recorded PIs also rose slightly in the second analysed period. We have proven the PIs prevalence increase in an ageing population.

Czech National Centre for Evidence Based Healthcare and Knowledge Translation (Cochrane Czech Republic Czech EBHC JBI Centre of Excellence Masaryk University GRADE Centre Institute of Biostatistics and Analyses Faculty of Medicine Masaryk University Brno Czech Republic

Department of Data Analyses Institute of Health Information and Statistics Prague Czech Republic

Department of Dermatology University Hospital Ostrava Ostrava Czech Republic

Department of Health Sciences Masaryk University Faculty of Medicine Brno Czech Republic

Department of Public Health Masaryk University Faculty of Medicine Brno Czech Republic

Research Unit of Plastic Surgery Department of Clinical Research Faculty of Health Sciences University of Southern Denmark Odense Denmark

School of Health Sciences Örebro University Örebro Sweden

School of Nursing and Midwifery Monash University Clayton Australia

School of Nursing and Midwifery Royal College of Surgeons in Ireland University of Medicine and Health Sciences Dublin Ireland

Skin Integrity Research Group University Centre for Nursing and Midwifery Department of Public Health and Primary Care Ghent University Ghent Belgium

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Changes in gene expression in pressure ulcers debrided by different approaches - a pilot study