Germline and somatic genetic variability of oxysterol-related genes in breast cancer patients with early disease of the luminal subtype
Jazyk angličtina Země Francie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
35525372
DOI
10.1016/j.biochi.2022.04.015
PII: S0300-9084(22)00110-9
Knihovny.cz E-zdroje
- Klíčová slova
- Breast cancer, Genomics, Mutation, Oxysterols, Prognosis, Variant,
- MeSH
- kohortové studie MeSH
- lidé MeSH
- nádory prsu * genetika patologie MeSH
- oxysteroly * metabolismus MeSH
- steroidní receptory * genetika MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- zárodečné buňky metabolismus MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- OSBPL2 protein, human MeSH Prohlížeč
- oxysteroly * MeSH
- steroidní receptory * MeSH
Oxysterols, oxidized derivatives of cholesterol, have been implicated in multiple pathologies, including cancer. In breast cancer, the link is especially strong due to interactions between oxysterols and estrogen receptor activity. Here, we provide the first dedicated study of 113 oxysterol-related genes in breast cancer patients of the luminal subtype, in terms of both their somatic and germline variability, using targeted high-throughput DNA sequencing of 100 normal-tumor pairs with very high coverage. In the full cohort, or subsets of patients stratified by therapy, we found 12 germline variants in ABCA1, ABCA8, ABCC1, GPR183, LDLR, MBTPS1, NR1I2, OSBPL2, OSBPL3, and OSBPL5 to associate with poor survival of patients and variants in ABCA8, ABCG2, and HSD3B7 (three in total) associated with better survival. However, no associations remained significant after correction for multiple tests. Analysis of somatic variants revealed significantly (after FDR correction) poorer survival in patients mutated in CYP46A1 and 9 interacting (according to STRING analysis) genes, as well as in OSBPL3 and a set of 20 genes that collectively associated with the progesterone receptor status of patients. We propose further exploration of these genes in an integrative manner together with gene expression and epigenomic data.
Aeskulab k s Prague Czech Republic
Biomedical Center Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
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