Multispectral imaging is used in various applications including astronomy, industry and agriculture. In retinal imaging, the single-shot multispectral image stack is typically acquired and analyzed. This multispectral analysis can provide information on various structural or metabolic properties. This paper describes the multispectral improvement of a video-ophthalmoscope, which can acquire retinal video sequences of the optic nerve head and peripapillary area using various spectral light illumination. The description of the multispectral video imaging is provided and several applications are described. These applications include multispectral retinal photoplethysmography, visualization of spontaneous vein pulsation and multispectral RGB image generation.

Department of Biomedical Engineering Faculty of Electrical Engineering and Communication Brno University of Technology Brno Czech Republic

Department of Ophthalmology and Optometry Masaryk University Brno Czech Republic

Department of Ophthalmology and Optometry St Ann University Hospital Brno Czech Republic

Department of Ophthalmology Friedrich Alexander University Erlangen Nürnberg Germany

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Assessment of retinal vein pulsation through video-ophthalmoscopy and simultaneous biosignals acquisition