An innovative amperometric immunosensor has been developed to detect antibiotic colistin from the chicken liver. Colistin is a antibacterial peptide that has been barred for human consumption, but it is being commonly used as a veterinary drug, and as a feed additive for livestock. In the present work, an immunosensor was developed by immobilizing an anti-colistin Ab onto the CNF/AuNPs surface of the screen-printed electrode. The sensor records electrochemical response in the chicken liver spiked with colistin with CV. Additionally, the characterization of electrode surface was done with FE-SEM, FTIR, and EIS at each step of fabrication. The lower LOD was 0.89 μgKg-1, with a R 2 of 0.901 using CV. Further validation of the immunosensor was conducted using commercial chicken liver samples, by comparing the results to those obtained using traditional methods. The fabricated immunosensor showed high specificity towards colistin, which remained stable for 6 months but with a 13% loss in the initial CV current.

Andalusian Research Institute in Data Science and Computational Intelligence University of Granada 18071 Granada Spain

Department of Biomedicine Faculty of Medicine University of Porto 4200 319 Porto Portugal

Department of Chemistry Faculty of Science University of Hradec Kralove 50003 Hradec Kralove Czech Republic

Faculty of Chemical and Food Technology Institute of Physical Chemistry and Chemical Physics Slovak University of Technology 81237 Bratislava Slovakia

Institute for Research and Innovation in Health University of Porto 4200 135 Porto Portugal

Institute of Research and Advanced Training in Health Sciences and Technologies Rua Central de Gandra 1317 4585 116 Gandra PRD Portugal

School of Bioengineering and Food Technology Shoolini University of Biotechnology and Management Sciences Solan 173229 India

Zoology Department College of Science King Saud University Riyadh 11451 Saudi Arabia

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