Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect the role of the environment (ionic conditions, protein and lipid compositions, membrane curvature) and the different partners associated with specific membrane reshaping processes, we undertake in vitro bottom-up approaches. In recent years, there has been keen interest in revealing the role of septin proteins associated with major diseases. Septins are essential and ubiquitous cytoskeletal proteins that interact with the plasma membrane. They are implicated in cell division, cell motility, neuro-morphogenesis, and spermiogenesis, among other functions. It is, therefore, important to understand how septins interact and organize at membranes to subsequently induce membrane deformations and how they can be sensitive to specific membrane curvatures. This article aims to decipher the interplay between the ultra-structure of septins at a molecular level and the membrane remodeling occurring at a micron scale. To this end, budding yeast, and mammalian septin complexes were recombinantly expressed and purified. A combination of in vitro assays was then used to analyze the self-assembly of septins at the membrane. Supported lipid bilayers (SLBs), giant unilamellar vesicles (GUVs), large unilamellar vesicles (LUVs), and wavy substrates were used to study the interplay between septin self-assembly, membrane reshaping, and membrane curvature.

Department of Bionanoscience Kavli Institute of Nanoscience Delft Delft University of Technology

Department of Chemical Engineering Imperial College London

Institut Fresnel CNRS UMR7249 Aix Marseille Univ Centrale Marseille

Laboratoire Matière et Systèmes Complexes Université Paris Cité;

Laboratoire Physico Chimie Curie Institut Curie PSL Research University Sorbonne Université

Laboratoire Physico Chimie Curie Institut Curie PSL Research University Sorbonne Université;

Laboratoire Physico Chimie Curie Institut Curie PSL Research University Sorbonne Université; Institute of Biotechnology Czech Academy of Sciences BIOCEV

Sorbonne Université CNRS Institut de Biologie Paris Seine

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