SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Cysteine, Global proteome, IodoTMT, Liquid chromatography, Mass spectrometry, Redox, SILAC,
- MeSH
- chromatografie kapalinová metody MeSH
- cystein metabolismus MeSH
- izotopové značení metody MeSH
- oxidace-redukce MeSH
- proteom * metabolismus MeSH
- proteomika * metody MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cystein MeSH
- proteom * MeSH
Stable isotope labeling by amino acids in cell culture (SILAC) and iodoacetyl tandem mass tag (iodoTMT) are well-implemented mass spectrometry-based approaches for quantification of proteins and for site-mapping of cysteine modification. We describe here a combination of SILAC and iodoTMT to assess ongoing changes in the global proteome and cysteine modification levels using liquid chromatography separation coupled with high-resolution mass spectrometry (LC-MS/MS).
BIOCEV 1st Faculty of Medicine Charles University Prague Czech Republic
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Yale Cancer Biology Institute Yale University West Haven CT USA
Zobrazit více v PubMed
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