SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cysteine, Global proteome, IodoTMT, Liquid chromatography, Mass spectrometry, Redox, SILAC,
- MeSH
- Chromatography, Liquid methods MeSH
- Cysteine metabolism MeSH
- Isotope Labeling methods MeSH
- Oxidation-Reduction MeSH
- Proteome * metabolism MeSH
- Proteomics * methods MeSH
- Tandem Mass Spectrometry methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cysteine MeSH
- Proteome * MeSH
Stable isotope labeling by amino acids in cell culture (SILAC) and iodoacetyl tandem mass tag (iodoTMT) are well-implemented mass spectrometry-based approaches for quantification of proteins and for site-mapping of cysteine modification. We describe here a combination of SILAC and iodoTMT to assess ongoing changes in the global proteome and cysteine modification levels using liquid chromatography separation coupled with high-resolution mass spectrometry (LC-MS/MS).
BIOCEV 1st Faculty of Medicine Charles University Prague Czech Republic
Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic
Yale Cancer Biology Institute Yale University West Haven CT USA
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